Laminin alpha 2 Antibody (2D4) - Azide and BSA Free

Novus Biologicals | Catalog # H00003908-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Sandwich ELISA

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2D4

Format

Azide and BSA Free
View all Laminin alpha 2 Primary Antibodies »

Product Specifications

Immunogen

LAMA2 (NP_000417, 3013 a.a. ~ 3122 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. DAGVPGHLCDGQWHKVTANKIKHRIELTVDGNQVEAQSPNPASTSADTNDPVFVGGFPDDLKQFGLTTSIPFRGCIRSLKLTKGTGKPLEVNFAKALELRGVQPVSCPAN

Localization

Extracellular

Specificity

LAMA2 - laminin, alpha 2 (merosin, congenital muscular dystrophy)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Laminin alpha 2 Antibody (2D4) - Azide and BSA Free

Immunohistochemistry-Paraffin: Laminin alpha 2 Antibody (2D4) [H00003908-M01]

Immunohistochemistry-Paraffin: Laminin alpha 2 Antibody (2D4) [H00003908-M01]

Immunohistochemistry-Paraffin: Laminin alpha 2 Antibody (2D4) [H00003908-M01] - Analysis of monoclonal antibody to LAMA2 on formalin-fixed paraffin-embedded human pancreas. Antibody concentration 1 ug/ml.
Sandwich ELISA: Laminin alpha 2 Antibody (2D4) [H00003908-M01] - Detection limit for recombinant GST tagged LAMA2 is 1 ng/ml as a capture antibody.

Applications for Laminin alpha 2 Antibody (2D4) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactivity against recombinant protein for WB. It has been used for IHC-P and ELISA. Use in Immunohistochemistry reported in scientific literature (PMID: 27390862).

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Laminin alpha 2

Laminin, an extracellular protein, is a major component of the basement membrane. It is thought to mediate the attachment, migration, and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. It is composed of three subunits, alpha, beta, and gamma, which are bound to each other by disulfide bonds into a cross-shaped molecule. This gene encodes the alpha 2 chain, which constitutes one of the subunits of laminin 2 (merosin) and laminin 4 (s-merosin). Mutations in this gene have been identified as the cause of congenital merosin-deficient muscular dystrophy. Two transcript variants encoding different proteins have been found for this gene.

Alternate Names

LAMA2, LAMM, Merosin

Entrez Gene IDs

3908 (Human)

Gene Symbol

LAMA2

UniProt

NP_000417 (Human)

Additional Laminin alpha 2 Products

Product Documents for Laminin alpha 2 Antibody (2D4) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Laminin alpha 2 Antibody (2D4) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Extracellular Matrix Molecules

Citations for Laminin alpha 2 Antibody (2D4) - Azide and BSA Free

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Protocols

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FAQs

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