Laminin alpha 2 Antibody (CL3450) - BSA Free

Novus Biologicals | Catalog # NBP2-46624

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL3450

Format

BSA Free
View all Laminin alpha 2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from P24043, with the exact immunogen sequence remaining proprietary.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Laminin alpha 2 Antibody (CL3450) - BSA Free

Immunohistochemistry-Paraffin: Laminin alpha 2 Antibody (CL3450) [NBP2-46624]

Immunohistochemistry-Paraffin: Laminin alpha 2 Antibody (CL3450) [NBP2-46624]

Immunohistochemistry-Paraffin: Laminin alpha 2 Antibody (CL3450) [NBP2-46624] - Laminin alpha 2 Antibody (3450) [NBP2-46624] - Staining in human heart muscle and tonsil tissues. Corresponding LAMA2 RNA-seq data are presented for the same tissues.
Western Blot: Laminin alpha 2 Antibody (CL3450) [NBP2-46624]

Western Blot: Laminin alpha 2 Antibody (CL3450) [NBP2-46624]

Western Blot: Laminin alpha 2 Antibody (3450) [NBP2-46624] - Analysis of purified human recombinant Laminin-211, Laminin-221, Laminin-332, Laminin-421, Laminin-511 and Laminin-121.
Immunohistochemistry-Paraffin: Laminin alpha 2 Antibody (CL3450) [NBP2-46624]

Immunohistochemistry-Paraffin: Laminin alpha 2 Antibody (CL3450) [NBP2-46624]

Immunohistochemistry-Paraffin: Laminin alpha 2 Antibody (3450) [NBP2-46624] - Staining of mouse heart muscle shows strong membranous positivity in cardiomyocytes.
Immunohistochemistry-Paraffin: Laminin alpha 2 Antibody (CL3450) [NBP2-46624]

Immunohistochemistry-Paraffin: Laminin alpha 2 Antibody (CL3450) [NBP2-46624]

Immunohistochemistry-Paraffin: Laminin alpha 2 Antibody (3450) [NBP2-46624] - Staining of rat stomach shows strong positivity in basement membrane of glandular cells.
Immunohistochemistry-Paraffin: Laminin alpha 2 Antibody (CL3450) [NBP2-46624]

Immunohistochemistry-Paraffin: Laminin alpha 2 Antibody (CL3450) [NBP2-46624]

Immunohistochemistry-Paraffin: Laminin alpha 2 Antibody (3450) [NBP2-46624] - Staining of rat heart muscle shows strong membranous positivity in cardiomyocytes.
Immunohistochemistry-Paraffin: Laminin alpha 2 Antibody (CL3450) [NBP2-46624]

Immunohistochemistry-Paraffin: Laminin alpha 2 Antibody (CL3450) [NBP2-46624]

Immunohistochemistry-Paraffin: Laminin alpha 2 Antibody (3450) [NBP2-46624] - Staining of human heart muscle shows strong membranous positivity in cardiomyocytes.
Immunohistochemistry-Paraffin: Laminin alpha 2 Antibody (CL3450) [NBP2-46624]

Immunohistochemistry-Paraffin: Laminin alpha 2 Antibody (CL3450) [NBP2-46624]

Immunohistochemistry-Paraffin: Laminin alpha 2 Antibody (3450) [NBP2-46624] - Staining of human placenta shows moderate positivity in basement membrane of trophoblastic cells.
Immunohistochemistry-Paraffin: Laminin alpha 2 Antibody (CL3450) [NBP2-46624]

Immunohistochemistry-Paraffin: Laminin alpha 2 Antibody (CL3450) [NBP2-46624]

Immunohistochemistry-Paraffin: Laminin alpha 2 Antibody (3450) [NBP2-46624] - Staining of human cerebral cortex shows moderate positivity in blood vessels.
Immunohistochemistry-Paraffin: Laminin alpha 2 Antibody (CL3450) [NBP2-46624]

Immunohistochemistry-Paraffin: Laminin alpha 2 Antibody (CL3450) [NBP2-46624]

Immunohistochemistry-Paraffin: Laminin alpha 2 Antibody (3450) [NBP2-46624] - Staining of human tonsil shows no positivity in lymphoid cells as expected.

Applications for Laminin alpha 2 Antibody (CL3450) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200-1:500

Western Blot

1 ug/ml
Application Notes
IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Laminin alpha 2

Laminin, an extracellular protein, is a major component of the basement membrane. It is thought to mediate the attachment, migration, and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. It is composed of three subunits, alpha, beta, and gamma, which are bound to each other by disulfide bonds into a cross-shaped molecule. This gene encodes the alpha 2 chain, which constitutes one of the subunits of laminin 2 (merosin) and laminin 4 (s-merosin). Mutations in this gene have been identified as the cause of congenital merosin-deficient muscular dystrophy. Two transcript variants encoding different proteins have been found for this gene.

Alternate Names

LAMA2, LAMM, Merosin

Entrez Gene IDs

3908 (Human)

Gene Symbol

LAMA2

UniProt

P24043

Additional Laminin alpha 2 Products

Product Documents for Laminin alpha 2 Antibody (CL3450) - BSA Free

Certificate of Analysis

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Product Specific Notices for Laminin alpha 2 Antibody (CL3450) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Extracellular Matrix Molecules

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Protocols

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