Laminin alpha 2 Antibody (CL3450) - BSA Free

Novus Biologicals | Catalog # NBP3-57831

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Mouse (96%), Rat (98%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG1 Clone # CL3450

Format

BSA Free
View all Laminin alpha 2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from P24043, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Laminin alpha 2 Antibody (CL3450) - BSA Free

Laminin alpha 2 Antibody (CL3450) - BSA Free

Laminin alpha 2 Antibody (CL3450) - BSA Free

Analysis in human heart muscle and tonsil tissues using NBP3-57831 antibody. Corresponding LAMA2 RNA-seq data are presented for the same tissues.
Laminin alpha 2 Antibody (CL3450) - BSA Free

Laminin alpha 2 Antibody (CL3450) - BSA Free

Analysis in human heart tissue.
Laminin alpha 2 Antibody (CL3450) - BSA Free

Laminin alpha 2 Antibody (CL3450) - BSA Free

Analysis of purified human recombinant Laminin-211, Laminin-221, Laminin-332, Laminin-421, Laminin-511 and Laminin-121.
Laminin alpha 2 Antibody (CL3450) - BSA Free

Laminin alpha 2 Antibody (CL3450) - BSA Free

Staining of human cerebral cortex shows moderate positivity in blood vessels.
Laminin alpha 2 Antibody (CL3450) - BSA Free

Laminin alpha 2 Antibody (CL3450) - BSA Free

Staining of human heart muscle shows strong membranous positivity in cardiomyocytes.
Laminin alpha 2 Antibody (CL3450) - BSA Free

Laminin alpha 2 Antibody (CL3450) - BSA Free

Staining of human placenta shows moderate positivity in basement membrane of trophoblastic cells.
Laminin alpha 2 Antibody (CL3450) - BSA Free

Laminin alpha 2 Antibody (CL3450) - BSA Free

Staining of human skeletal muscle shows moderate membranous positivity in myocytes.
Laminin alpha 2 Antibody (CL3450) - BSA Free

Laminin alpha 2 Antibody (CL3450) - BSA Free

Staining of human tonsil shows no positivity in lymphoid cells as expected.
Laminin alpha 2 Antibody (CL3450) - BSA Free

Laminin alpha 2 Antibody (CL3450) - BSA Free

Staining of mouse heart muscle shows strong membranous positivity in cardiomyocytes.
Laminin alpha 2 Antibody (CL3450) - BSA Free

Laminin alpha 2 Antibody (CL3450) - BSA Free

Staining of mouse testis shows moderate positivity in basement membrane of seminiferous ducts and Leydig cells.
Laminin alpha 2 Antibody (CL3450) - BSA Free

Laminin alpha 2 Antibody (CL3450) - BSA Free

Staining of rat heart muscle shows strong membranous positivity in cardiomyocytes.
Laminin alpha 2 Antibody (CL3450) - BSA Free

Laminin alpha 2 Antibody (CL3450) - BSA Free

Staining of rat skeletal muscle shows moderate membranous positivity in myocytes.
Laminin alpha 2 Antibody (CL3450) - BSA Free

Laminin alpha 2 Antibody (CL3450) - BSA Free

Staining of rat stomach shows strong positivity in basement membrane of glandular cells.

Applications for Laminin alpha 2 Antibody (CL3450) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 9 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2), 40% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Laminin alpha 2

Laminin, an extracellular protein, is a major component of the basement membrane. It is thought to mediate the attachment, migration, and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. It is composed of three subunits, alpha, beta, and gamma, which are bound to each other by disulfide bonds into a cross-shaped molecule. This gene encodes the alpha 2 chain, which constitutes one of the subunits of laminin 2 (merosin) and laminin 4 (s-merosin). Mutations in this gene have been identified as the cause of congenital merosin-deficient muscular dystrophy. Two transcript variants encoding different proteins have been found for this gene.

Alternate Names

LAMA2, LAMM, Merosin

Gene Symbol

LAMA2

Additional Laminin alpha 2 Products

Product Documents for Laminin alpha 2 Antibody (CL3450) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Laminin alpha 2 Antibody (CL3450) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Extracellular Matrix Molecules

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Protocols

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