Laminin alpha 4 Antibody (CL3183) - Azide and BSA Free
Novus Biologicals | Catalog # NBP3-57878
Recombinant Monoclonal Antibody
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Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Mouse IgG2B Clone # CL3183
Format
Azide and BSA Free
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Product Specifications
Immunogen
This antibody was developed using a recombinant protein derived from Q16363, with the exact immunogen sequence remaining proprietary.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Laminin alpha 4 Antibody (CL3183) - Azide and BSA Free
Laminin alpha 4 Antibody (CL3183) - BSA Free
Analysis in human placenta tissue.
Laminin alpha 4 Antibody (CL3183) - BSA Free
Analysis in human placenta tissue.
Laminin alpha 4 Antibody (CL3183) - BSA Free
Analysis of purified human recombinant Laminin-411, Laminin-421, Laminin-511, Laminin-121, Laminin-221 and Laminin-332.
Laminin alpha 4 Antibody (CL3183) - BSA Free
Staining of human colon shows strong positivity in basement membrane of glandular cells.
Laminin alpha 4 Antibody (CL3183) - BSA Free
Staining of human fallopian tube shows moderate positivity in basement membrane of glandular cells, as well as moderate membranous positivity in blood vessels.
Laminin alpha 4 Antibody (CL3183) - BSA Free
Staining of human lymph node shows no positivity in non-germinal center cells as expected.
Laminin alpha 4 Antibody (CL3183) - BSA Free
Staining of human placenta shows strong positivity in basement membrane of trophoblastic cells and endothelial cells.
Laminin alpha 4 Antibody (CL3183) - BSA Free
Staining of human prostate shows moderate to strong membranous positivity in smooth muscle cells.
Laminin alpha 4 Antibody (CL3183) - BSA Free
Staining of mouse adrenal gland shows moderate membranous positivity in glandular cells.
Laminin alpha 4 Antibody (CL3183) - BSA Free
Staining of mouse epididymis shows moderate to strong membranous positivity in smooth muscle cells.
Laminin alpha 4 Antibody (CL3183) - BSA Free
Staining of mouse stomach shows moderate positivity in basement membrane of glandular cells.
Laminin alpha 4 Antibody (CL3183) - BSA Free
Staining of rat heart muscle shows moderate to strong membranous positivity in cardiomyocytes.
Laminin alpha 4 Antibody (CL3183) - BSA Free
Staining of rat kidney shows strong membranous positivity in cells in glomeruli.
Laminin alpha 4 Antibody (CL3183) - BSA Free
Staining of rat lung shows moderate membranous positivity in pneumocytes.
Laminin alpha 4 Antibody (CL3183) - BSA Free
Staining of rat stomach shows moderate to strong positivity in basement membrane of glandular cells.Applications for Laminin alpha 4 Antibody (CL3183) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200 - 1:500
Western Blot
1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Formulation, Preparation, and Storage
Purification
Protein A purified
Reconstitution
Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.
Formulation
Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
Format
Azide and BSA Free
Preservative
0.02% Sodium Azide
Concentration
LYOPH mg/ml
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Calculators
Background: Laminin alpha 4
Alternate Names
LAMA4
Gene Symbol
LAMA4
Additional Laminin alpha 4 Products
Product Documents for Laminin alpha 4 Antibody (CL3183) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Laminin alpha 4 Antibody (CL3183) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
