Laminin alpha 4 Antibody (CL3185) - Azide and BSA Free
Novus Biologicals | Catalog # NBP3-44409
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Human, Mouse
Applications
Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # CL3185
Format
Azide and BSA Free
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Product Specifications
Immunogen
This antibody was generated using a recombinant protein sequence of Q16363, with the exact immunogen sequence remaining proprietary.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Description
Novus Biologicals Mouse Laminin alpha 4 Antibody (CL3185) - Azide and BSA Free (NBP2-42393) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for Laminin alpha 4 Antibody (CL3185) - Azide and BSA Free
![Laminin alpha 4 Antibody (CL3185) - Azide and BSA Free Immunohistochemistry-Paraffin: Laminin alpha 4 Antibody [NBP3-44409]](https://beta-resources.rndsystems.com/images/products/nbp3-44409_mouse-laminin-alpha-4-mab-cl3185-azide-and-bsa-free-282202512121911.jpg "Immunohistochemistry-Paraffin: Laminin alpha 4 Antibody [NBP3-44409]")
![Laminin alpha 4 Antibody (CL3185) - Azide and BSA Free Immunohistochemistry-Paraffin: Laminin alpha 4 Antibody [NBP3-44409]](https://beta-resources.rndsystems.com/images/products/nbp3-44409_mouse-laminin-alpha-4-mab-cl3185-azide-and-bsa-free-282202512384216.jpg "Immunohistochemistry-Paraffin: Laminin alpha 4 Antibody [NBP3-44409]")
Immunohistochemistry-Paraffin: Laminin alpha 4 Antibody [NBP3-44409]
Staining of human colon shows strong positivity in basement membrane of glandular cells.![Laminin alpha 4 Antibody (CL3185) - Azide and BSA Free Immunohistochemistry-Paraffin: Laminin alpha 4 Antibody [NBP3-44409]](https://beta-resources.rndsystems.com/images/products/nbp3-44409_mouse-laminin-alpha-4-mab-cl3185-azide-and-bsa-free-282202513135978.jpg "Immunohistochemistry-Paraffin: Laminin alpha 4 Antibody [NBP3-44409]")
Immunohistochemistry-Paraffin: Laminin alpha 4 Antibody [NBP3-44409]
Staining of human lymph node shows no positivity in lymphoid cells as expected.![Laminin alpha 4 Antibody (CL3185) - Azide and BSA Free Immunohistochemistry-Paraffin: Laminin alpha 4 Antibody [NBP3-44409]](https://beta-resources.rndsystems.com/images/products/nbp3-44409_mouse-laminin-alpha-4-mab-cl3185-azide-and-bsa-free-28220251344259.jpg "Immunohistochemistry-Paraffin: Laminin alpha 4 Antibody [NBP3-44409]")
Immunohistochemistry-Paraffin: Laminin alpha 4 Antibody [NBP3-44409]
Staining of human placenta shows strong membranous positivity in endothelial cells.![Laminin alpha 4 Antibody (CL3185) - Azide and BSA Free Immunohistochemistry-Paraffin: Laminin alpha 4 Antibody [NBP3-44409]](https://beta-resources.rndsystems.com/images/products/nbp3-44409_mouse-laminin-alpha-4-mab-cl3185-azide-and-bsa-free-28220251324537.jpg "Immunohistochemistry-Paraffin: Laminin alpha 4 Antibody [NBP3-44409]")
Immunohistochemistry-Paraffin: Laminin alpha 4 Antibody [NBP3-44409]
Staining of human smooth muscle in prostate shows strong membranous positivity in myocytes.![Laminin alpha 4 Antibody (CL3185) - Azide and BSA Free Western Blot: Laminin alpha 4 Antibody [NBP3-44409]](https://beta-resources.rndsystems.com/images/products/nbp3-44409_mouse-laminin-alpha-4-mab-cl3185-azide-and-bsa-free-28220251337885.jpg "Western Blot: Laminin alpha 4 Antibody [NBP3-44409]")
Western Blot: Laminin alpha 4 Antibody [NBP3-44409]
Analysis of purified human recombinant Laminin-411, Laminin-421, Laminin-511, Laminin-121, Laminin-221 and Laminin-332.![Laminin alpha 4 Antibody (CL3185) - Azide and BSA Free Immunohistochemistry-Paraffin: Laminin alpha 4 Antibody [NBP3-44409]](https://beta-resources.rndsystems.com/images/products/nbp3-44409_mouse-laminin-alpha-4-mab-cl3185-azide-and-bsa-free-282202513261938.jpg "Immunohistochemistry-Paraffin: Laminin alpha 4 Antibody [NBP3-44409]")
Immunohistochemistry-Paraffin: Laminin alpha 4 Antibody [NBP3-44409]
Staining of mouse heart muscle shows strong membranous positivity in cardiomyocytes.![Laminin alpha 4 Antibody (CL3185) - Azide and BSA Free Immunohistochemistry-Paraffin: Laminin alpha 4 Antibody [NBP3-44409]](https://beta-resources.rndsystems.com/images/products/nbp3-44409_mouse-laminin-alpha-4-mab-cl3185-azide-and-bsa-free-282202513381517.jpg "Immunohistochemistry-Paraffin: Laminin alpha 4 Antibody [NBP3-44409]")
Immunohistochemistry-Paraffin: Laminin alpha 4 Antibody [NBP3-44409]
Staining of mouse stomach shows strong positivity in basement membrane in glandular cells, as well as membranous positivity in smooth muscle cells.![Laminin alpha 4 Antibody (CL3185) - Azide and BSA Free Immunohistochemistry-Paraffin: Laminin alpha 4 Antibody [NBP3-44409]](https://beta-resources.rndsystems.com/images/products/nbp3-44409_mouse-laminin-alpha-4-mab-cl3185-azide-and-bsa-free-282202513245469.jpg "Immunohistochemistry-Paraffin: Laminin alpha 4 Antibody [NBP3-44409]")
Immunohistochemistry-Paraffin: Laminin alpha 4 Antibody [NBP3-44409]
Staining of mouse epididymis shows strong membranous positivity in smooth muscle cells.Applications for Laminin alpha 4 Antibody (CL3185) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:500 - 1:1000
Western Blot
1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Formulation, Preparation, and Storage
Purification
Protein A purified
Reconstitution
Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.
Formulation
Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Calculators
Background: Laminin alpha 4
Alternate Names
LAMA4
Gene Symbol
LAMA4
Additional Laminin alpha 4 Products
Product Documents for Laminin alpha 4 Antibody (CL3185) - Azide and BSA Free
Product Specific Notices for Laminin alpha 4 Antibody (CL3185) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
Blood-Brain Barrier Pathway: Anatomy
