Laminin Antibody [Alexa Fluor® 647]

Novus Biologicals | Catalog # NB300-144AF647

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Chinese Hamster, Invertebrate, Mammal, Rabbit, Sheep

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunohistochemistry Free-Floating, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Frozen, Immunocytochemistry/ Immunofluorescence

Label

Alexa Fluor 647 (Excitation = 650 nm, Emission = 668 nm)

Antibody Source

Polyclonal Rabbit IgG
View all Laminin Primary Antibodies »

Product Specifications

Immunogen

Laminin Antibody was made to Laminin 111 isolated from mouse Engelbreth-Holm-Swarm (EHS) sarcoma cells. [UniProt# P19137]

Reactivity Notes

Rabbit, Fruit Bat, Chinese Hamster, and S. mansoni reactivity reported in scientific literature (PMID: 18214989, 31877588, 29251349, and 28114363 respectively). Human, Mouse, Rat, and Sheep reported in multiple pieces of scientific literature.

Localization

Secreted, Basement membrane, Extracellular matrix

Marker

Basement Membrane Marker

Specificity

Laminin Antibody is pan-specific and reacts well with all Laminin isoforms tested: Laminin-1 (alpha-1, beta-1, and gamma-1) and Laminin-2 (alpha-2, beta-1, and gamma-1).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Laminin Antibody [Alexa Fluor® 647]

Frozen Mouse Tissue Stained for Laminin

Frozen Mouse Tissue Stained for Laminin

Laminin-Antibody-Alexa-Fluor-R-647-Immunohistochemistry-NB300-144AF647-img0003.jpg
Flow Cytometry of HeLa Cells Stained with Conjugated Laminin Antibody

Flow Cytometry of HeLa Cells Stained with Conjugated Laminin Antibody

A surface stain was performed on HeLa cells with Laminin Antibody NB300-144AF647 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.
Laminin Antibody [Alexa Fluor® 647]

Immunocytochemistry/ Immunofluorescence: Laminin Antibody [Alexa Fluor® 647] [NB300-144AF647] -

Immunocytochemistry/ Immunofluorescence: Laminin Antibody [Alexa Fluor® 647] [NB300-144AF647] - SOX17 regulates adult muscle regeneration after injury in Pax7CreERT2/+;Sox17fl/fl mutant mice.(A) Schematic outline of experimental procedure for tamoxifen (TMX) injection (i.p., intraperitoneal). CTX, cardiotoxin injection; d, days. (B) Representative images of cryosections from regenerating adult TA muscles d7 after injury, showing immunofluorescence for PAX7+ (quiescent, arrows) & PH3+PAX7+ (proliferating, arrowheads) cells. Scale bar, 25 μm. (C–D) Quantification of satellite cells as illustrated in (B). (E) Schematic outline of experimental procedure for TMX diet. CTX, cardiotoxin injection; d, days. (F) Representative images of cryosections from regenerating adult TA muscles d28 after injury, showing immunofluorescence for PAX7+ (quiescent, arrows) cells. Scale bar, 25 µm. (G) Quantification of satellite cells as illustrated in (F). (H–I) Quantification of cross-sectional area in µm2 (H) & myofiber number per mm2 (I). (J–K) Quantification of fat infiltration (Oil red O) (J) & fibrosis (Sirius red) (K) indicated as proportion of stained section (average of five sections per muscle). (L) Representative images of histological characterization of adult TA muscles 28 days after injury w/ Hematoxylin & eosin (cell infiltration; upper panel), Oil red O (fat infiltration; middle panel), & Sirius red (fibrosis; bottom panel) staining. Scale bars, 100 µm. CTRL, Sox17fl/fl; cKO, Pax7CreERT2/+;Sox17fl/fl. n ≥ 3 mice (each quantified at least in triplicate) for all experiments. Data expressed as mean ± s.e.m., statistically analyzed w/ Student’s unpaired t-test (C,D,G) & Mann-Whitney ranking test (H–K): n.s., not significant; *, p<0.05; **, p<0.01; ***, p<0.001, compared to CTRL. Image collected & cropped by CiteAb from following publication (https://pubmed.ncbi.nlm.nih.gov/29882512), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Laminin Antibody [Alexa Fluor® 647]

Immunocytochemistry/ Immunofluorescence: Laminin Antibody [Alexa Fluor® 647] [NB300-144AF647] -

Immunocytochemistry/ Immunofluorescence: Laminin Antibody [Alexa Fluor® 647] [NB300-144AF647] - SOX17 is necessary to maintain satellite cell quiescence in adult muscles.(A,F) Representative Soleus cryosection images showing immunofluorescence for satellite cells (PAX7+, arrows) in Pax3Cre/+;Sox17GFP/fl & Pax7CreERT2/+;Sox17fl/fl mice, with appropriate controls. Scale bars, 25 μm. Fibers are identified by LAMININ & nuclei are counterstained with DAPI. (B,G) Quantification of satellite cell number during postnatal growth (P14) & in adult. (C) Quantification of the ratio PAX7/MYOD+ satellite cells in P14 Soleus cryosections. (D) RT-qPCR analysis on adult TA muscles for Pax7 & SoxF genes in fresh FACS-isolated satellite cells from control & Sox17-knockout mice. (A–D) CTRL, Sox17GFP/fl; KO, Pax3Cre/+;Sox17GFP/fl. (E) Schematic outline of the experimental procedure for tamoxifen (TMX) injection (i.p., intraperitoneal) in Sox17fl/fl (CTRL) & Pax7CreERT2/+;Sox17fl/fl (cKO) mice. d, days. (E–G) CTRL, Sox17fl/fl; cKO, Pax7CreERT2/+;Sox17fl/fl. Quantification was performed in whole cross-sections. n ≥ 4 mice (each quantified in triplicate) for all experiments. Data expressed as mean ± s.e.m., statistically analyzed with Student’s unpaired t-test: *, p<0.05; **, p<0.01, compared to CTRL.Satellite cells characterization of control & Sox17-knockout mice.(A) Immunofluorescence of satellite cells (MCAD; M-cadherin) in adult Soleus cryosections from control & Sox17 mutant mice. Scale bar, 25 μm. (B) Quantification of satellite cell number illustrated in (A). CTRL, Sox17GFP/fl; KO, Pax3Cre/+;Sox17GFP/fl. n ≥ 4 mice (each quantified in triplicate) for all experiments. Data expressed as mean ± s.e.m., statistically analyzed with Student’s unpaired t-test: *, p<0.05, compared to CTRL. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29882512), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Laminin Antibody [Alexa Fluor® 647]

Immunocytochemistry/ Immunofluorescence: Laminin Antibody [Alexa Fluor® 647] [NB300-144AF647] -

Immunocytochemistry/ Immunofluorescence: Laminin Antibody [Alexa Fluor® 647] [NB300-144AF647] - SOX17 is necessary to maintain satellite cell quiescence in adult muscles.(A,F) Representative Soleus cryosection images showing immunofluorescence for satellite cells (PAX7+, arrows) in Pax3Cre/+;Sox17GFP/fl & Pax7CreERT2/+;Sox17fl/fl mice, with appropriate controls. Scale bars, 25 μm. Fibers are identified by LAMININ & nuclei are counterstained with DAPI. (B,G) Quantification of satellite cell number during postnatal growth (P14) & in adult. (C) Quantification of the ratio PAX7/MYOD+ satellite cells in P14 Soleus cryosections. (D) RT-qPCR analysis on adult TA muscles for Pax7 & SoxF genes in fresh FACS-isolated satellite cells from control & Sox17-knockout mice. (A–D) CTRL, Sox17GFP/fl; KO, Pax3Cre/+;Sox17GFP/fl. (E) Schematic outline of the experimental procedure for tamoxifen (TMX) injection (i.p., intraperitoneal) in Sox17fl/fl (CTRL) & Pax7CreERT2/+;Sox17fl/fl (cKO) mice. d, days. (E–G) CTRL, Sox17fl/fl; cKO, Pax7CreERT2/+;Sox17fl/fl. Quantification was performed in whole cross-sections. n ≥ 4 mice (each quantified in triplicate) for all experiments. Data expressed as mean ± s.e.m., statistically analyzed with Student’s unpaired t-test: *, p<0.05; **, p<0.01, compared to CTRL.Satellite cells characterization of control & Sox17-knockout mice.(A) Immunofluorescence of satellite cells (MCAD; M-cadherin) in adult Soleus cryosections from control & Sox17 mutant mice. Scale bar, 25 μm. (B) Quantification of satellite cell number illustrated in (A). CTRL, Sox17GFP/fl; KO, Pax3Cre/+;Sox17GFP/fl. n ≥ 4 mice (each quantified in triplicate) for all experiments. Data expressed as mean ± s.e.m., statistically analyzed with Student’s unpaired t-test: *, p<0.05, compared to CTRL. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29882512), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Laminin Antibody [Alexa Fluor® 647]

Immunocytochemistry/ Immunofluorescence: Laminin Antibody [Alexa Fluor® 647] [NB300-144AF647] -

Immunocytochemistry/ Immunofluorescence: Laminin Antibody [Alexa Fluor® 647] [NB300-144AF647] - MuSC-specific Cdkn1c ablation hinders muscle regeneration.(A) Time-course of tamoxifen administration, intramuscular injury of TA muscle (CTX arrow), & muscle harvest (D7 arrow). (B–G) Cryosections of TA muscle were stained for histological & satellite cell population characterization 7 days after CTX injection. Analyzed animals at (B-G) were wild-type littermates (Wt; Pax7+; Cdkn1c+; B–G), Cre control (Pax7CreERT2; B’–G’), & Cdkn1c cKO (Pax7CreERT2; Cdkn1cFlox; B’’–G’’). (B) HE staining for histologic characterization of the muscles. (C) Oil Red O staining for evaluation of fat infiltration of the muscles. (D–E) embryonic myosin (eMYHC, red)/LAMININ (LAM, green) immunofluorescence to mark newly formed myofibers post-regeneration. (F–G) PAX7 (red)/LAMININ (LAM, green) immunofluorescence to mark PAX7+ satellite cells. Nuclei in (D-G) were counter-stained with DAPI (blue). Scale bars, 50 μm. (H) Quantification of (F-G). Data show mean +SD, n ≥ 5 animals. Asterisks indicate significance; **p≤0.01.In vivo MuSC-specific Cdkn1c ablation. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30284969), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Laminin Antibody [Alexa Fluor® 647]

Immunocytochemistry/ Immunofluorescence: Laminin Antibody [Alexa Fluor® 647] [NB300-144AF647] -

Immunocytochemistry/ Immunofluorescence: Laminin Antibody [Alexa Fluor® 647] [NB300-144AF647] - MuSC-specific Cdkn1c ablation hinders muscle regeneration.(A) Time-course of tamoxifen administration, intramuscular injury of TA muscle (CTX arrow), & muscle harvest (D7 arrow). (B–G) Cryosections of TA muscle were stained for histological & satellite cell population characterization 7 days after CTX injection. Analyzed animals at (B-G) were wild-type littermates (Wt; Pax7+; Cdkn1c+; B–G), Cre control (Pax7CreERT2; B’–G’), & Cdkn1c cKO (Pax7CreERT2; Cdkn1cFlox; B’’–G’’). (B) HE staining for histologic characterization of the muscles. (C) Oil Red O staining for evaluation of fat infiltration of the muscles. (D–E) embryonic myosin (eMYHC, red)/LAMININ (LAM, green) immunofluorescence to mark newly formed myofibers post-regeneration. (F–G) PAX7 (red)/LAMININ (LAM, green) immunofluorescence to mark PAX7+ satellite cells. Nuclei in (D-G) were counter-stained with DAPI (blue). Scale bars, 50 μm. (H) Quantification of (F-G). Data show mean +SD, n ≥ 5 animals. Asterisks indicate significance; **p≤0.01.In vivo MuSC-specific Cdkn1c ablation. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30284969), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Laminin Antibody [Alexa Fluor® 647]

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry Free-Floating

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This Laminin antibody can be used for Immunocytochemistry/Immunofluorescence, Functional assays, Immunohistochemistry paraffin and frozen sections, and Western blotting where it detects bands at 200 and 400 kDa. Immunostaining is enhanced by antigen retrieval with pepsin, especially paraffin tissue.

Spectra Viewer

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Laminin

Laminin, the most abundant structural and biologically active component in basement membranes, is a complex extracellular glycoprotein with high molecular weight (900 kDa). Laminin is composed of a laminin alpha (400 kDa), beta (215 kDa) and gamma chain (205 kDa), whose multidomain proteins are encoded by distinct genes (LAMA, LAMB, LAMC respectively) and have several isoforms of each chain. The biological functions of the different chains and trimer molecules are largely unknown, but some of the chains have been shown to differ with respect to their tissue distribution, reflecting diverse functions in vivo. Laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.

Laminins are an important and biologically active part of the basal lamina, influencing cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis, where anti-laminin antibodies can be widely used to label blood vessels and basement membranes (1). Significant quantities of laminin are found in basement membranes, the thin extracellular matrices that surround epithelial tissue, nerve, fat cells and smooth, striated and cardiac muscle. Excessive serum laminin levels have been associated with fibrosis, cirrhosis and hepatitis, serious and frequent complications of chronic active liver disease characterized by excessive deposition of various normal components of connective tissue in liver (2). Epithelial mesenchymal transition (EMT) biomarkers include fibronectin, laminin, N-cadherin, and Slug (3).

References

1. Yang, M. Y., Chiao, M. T., Lee, H. T., Chen, C. M., Yang, Y. C., Shen, C. C., & Ma, H. I. (2015). An innovative three-dimensional gelatin foam culture system for improved study of glioblastoma stem cell behavior. J Biomed Mater Res B Appl Biomater, 103(3), 618-628. doi:10.1002/jbm.b.33214

2. Mak, K. M., & Mei, R. (2017). Basement Membrane Type IV Collagen and Laminin: An Overview of Their Biology and Value as Fibrosis Biomarkers of Liver Disease. Anat Rec (Hoboken), 300(8), 1371-1390. doi:10.1002/ar.23567

3. Choi, S., Yu, J., Park, A., Dubon, M. J., Do, J., Kim, Y.,... Park, K. S. (2019). BMP-4 enhances epithelial mesenchymal transition and cancer stem cell properties of breast cancer cells via Notch signaling. Sci Rep, 9(1), 11724. doi:10.1038/s41598-019-48190-5

Alternate Names

LAMA, Laminin A chain, laminin subunit alpha-1, laminin, alpha 1, Laminin-1 subunit alpha, Laminin-3 subunit alpha, PTBHS, S-LAM alpha, S-laminin subunit alpha

Gene Symbol

LAMA1

Additional Laminin Products

Product Documents for Laminin Antibody [Alexa Fluor® 647]

Certificate of Analysis

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Product Specific Notices for Laminin Antibody [Alexa Fluor® 647]

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Laminin Antibody [Alexa Fluor® 647]

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Laminin Antibody [Alexa Fluor® 647]

Showing  1 - 4 of 4 FAQs Showing All

  • Q: I'm looking for a basement membrane marker, can you suggest a Laminin antibody that can be used for this purpose?

    A: The following Laminin antibodies are Basement Membrane markers: NB120-17107, NB300-144, NB600-883, and NBP1-51759.

  • Q: If this product is used in an application or species as a part of a customer review, will that validate this product in the application/species?

    A: If any of our primary antibodes are used in an untested application or species and it is shown to work through images from customer reviews or through publications, this validates the application/species for this product. Please check out our Innovator's Reward Program if you decide to test an antibody with a species or application that is not currently listed.

  • Q: What is the theoretical molecular weight for your Laminin antibodies?

    A: The theoretical molecular weight for Laminin antibodies is 337 kDa.

  • Q: What research areas can this product be used in?

    A: All Laminin products can be used in: Angiogenesis, Apoptosis, Cancer, Cell Biology, Cellular Markers, Extracellular Matrix, Neuroscience, Signal Transduction, Tumor Suppressors.

  • Q: I'm looking for a basement membrane marker, can you suggest a Laminin antibody that can be used for this purpose?

    A: The following Laminin antibodies are Basement Membrane markers: NB120-17107, NB300-144, NB600-883, and NBP1-51759.

  • Q: If this product is used in an application or species as a part of a customer review, will that validate this product in the application/species?

    A: If any of our primary antibodes are used in an untested application or species and it is shown to work through images from customer reviews or through publications, this validates the application/species for this product. Please check out our Innovator's Reward Program if you decide to test an antibody with a species or application that is not currently listed.

  • Q: What is the theoretical molecular weight for your Laminin antibodies?

    A: The theoretical molecular weight for Laminin antibodies is 337 kDa.

  • Q: What research areas can this product be used in?

    A: All Laminin products can be used in: Angiogenesis, Apoptosis, Cancer, Cell Biology, Cellular Markers, Extracellular Matrix, Neuroscience, Signal Transduction, Tumor Suppressors.

  • Q: I'm looking for a basement membrane marker, can you suggest a Laminin antibody that can be used for this purpose?

    A: The following Laminin antibodies are Basement Membrane markers: NB120-17107, NB300-144, NB600-883, and NBP1-51759.

  • Q: If this product is used in an application or species as a part of a customer review, will that validate this product in the application/species?

    A: If any of our primary antibodes are used in an untested application or species and it is shown to work through images from customer reviews or through publications, this validates the application/species for this product. Please check out our Innovator's Reward Program if you decide to test an antibody with a species or application that is not currently listed.

  • Q: What is the theoretical molecular weight for your Laminin antibodies?

    A: The theoretical molecular weight for Laminin antibodies is 337 kDa.

  • Q: What research areas can this product be used in?

    A: All Laminin products can be used in: Angiogenesis, Apoptosis, Cancer, Cell Biology, Cellular Markers, Extracellular Matrix, Neuroscience, Signal Transduction, Tumor Suppressors.

  • Q: I'm looking for a basement membrane marker, can you suggest a Laminin antibody that can be used for this purpose?

    A: The following Laminin antibodies are Basement Membrane markers: NB120-17107, NB300-144, NB600-883, and NBP1-51759.

  • Q: If this product is used in an application or species as a part of a customer review, will that validate this product in the application/species?

    A: If any of our primary antibodes are used in an untested application or species and it is shown to work through images from customer reviews or through publications, this validates the application/species for this product. Please check out our Innovator's Reward Program if you decide to test an antibody with a species or application that is not currently listed.

  • Q: What is the theoretical molecular weight for your Laminin antibodies?

    A: The theoretical molecular weight for Laminin antibodies is 337 kDa.

  • Q: What research areas can this product be used in?

    A: All Laminin products can be used in: Angiogenesis, Apoptosis, Cancer, Cell Biology, Cellular Markers, Extracellular Matrix, Neuroscience, Signal Transduction, Tumor Suppressors.

Showing  1 - 4 of 4 FAQs Showing All
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