Laminin gamma 1 Antibody (A5)

Novus Biologicals | Catalog # NBP2-44751

Novus Biologicals

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Bovine

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Flow Cytometry, Immunofluorescence, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Frozen, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2a Kappa Clone # A5
View all Laminin gamma 1 Primary Antibodies »

Product Specifications

Immunogen

Murine EHS laminin preparation

Reactivity Notes

Bovine reported by a verified customer review.

Localization

Basement membrane

Specificity

Laminins are large hetero-trimeric, non-collagenous glycoproteins composed of alpha, beta, and gamma chains. This monoclonal antibody reacts with laminin B2/1 chain of ~210kDa and does not cross-react with other basement membrane components or fibronectin. Its specificity was established by immunoprecipitation and immunofluorescence of human skeletal muscle and kidney with laminin chain-specific monoclonal antibodys. Epithelial sheets in vivo are separated from the mesenchymal elements of the stroma by a thin layer of a specialized type of extracellular matrix termed the basement membrane (BM). This structure consists of individual components, some of which are ubiquitous in BMs and some are not. The ubiquitous ones comprise laminin (LN), entactin/nidogen (EN), collagen type IV (CIV), and large heparan sulfate proteoglycan (HSPG), which interact specifically with each other to form a continuous and regular BM. Alterations of BM integrity, from local discontinuities up to complete loss, are described in many types of human and animal epithelial neoplasms. This monoclonal antibody stains uniformly all human and murine basement membranes.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2a Kappa

Theoretical MW

210 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0 mg/ml. (NBP2-47842)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for Laminin gamma 1 Antibody (A5)

Immunohistochemistry-Paraffin: Laminin gamma 1 Antibody (A5) [NBP2-44751]

Immunohistochemistry-Paraffin: Laminin gamma 1 Antibody (A5) [NBP2-44751]

Immunohistochemistry-Paraffin: Laminin gamma 1 Antibody (A5) [NBP2-44751] - Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with Laminin gamma 1 Antibody (A5).
Immunohistochemistry-Frozen: Laminin gamma 1 Antibody (A5) [NBP2-44751]

Immunohistochemistry-Frozen: Laminin gamma 1 Antibody (A5) [NBP2-44751]

Immunohistochemistry-Frozen: Laminin gamma 1 Antibody (A5) [NBP2-44751] - Bovine frozen skeletal muscle section was fixed in 4% PFA for 5 min and stained with anti-laminin antibody (NBP2-44751) overnight at 4 C and then stained with a goat anti-rat-Alexa Fluor 488 secondary antibody (green) for 1 hour at room temperature. Section was then mounted in a mounting medium containing Dapi (blue). Image from verified customer review.
Immunohistochemistry-Paraffin: Laminin gamma 1 Antibody (A5) [NBP2-44751]

Immunohistochemistry-Paraffin: Laminin gamma 1 Antibody (A5) [NBP2-44751]

Immunohistochemistry-Paraffin: Laminin gamma 1 Antibody (A5) [NBP2-44751] - Human Renal Cell Carcinoma stained with Laminin Monoclonal Antibody (A5).

Applications for Laminin gamma 1 Antibody (A5)

Application
Recommended Usage

Flow Cytometry

0.5-1 ug/million cells

Immunocytochemistry/ Immunofluorescence

0.5-1.0 ug/ml

Immunofluorescence

1 - 2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Reviewed Applications

Read 1 review rated 5 using NBP2-44751 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Laminin gamma 1

Laminins are essential and abundant structural non-collagenous glycoproteins localizing to basement membranes. Basement membranes (cell-associated extracellular matrices (ECMs)) are polymers of Laminins with stabilizing Type IV Collagen networks, Nidogen and several proteoglycans. Basement membranes are found under epithelial layers, around the endothelium of blood vessels, and surrounding muscle, peripheral nerve and fat cells. Formation of basement membranes influences cell proliferation, phenotype, migration, gene expression and tissue architecture. Each Laminin is a heterotrimer of alpha, beta and gamma chain subunits that undergoes cell-secretion and incorporation into the ECM. Laminins can self-assemble, bind to other matrix macromolecules and have unique and shared cell interactions mediated by Integrins, dystroglycan and cognate Laminin receptors. The human Laminin gamma-1 gene maps to chromosome 1q31 and is ubiquitously expressed in tissues that produce basement membranes.

Alternate Names

LAMC1, Laminin B2

Gene Symbol

LAMC1

UniProt

P11047 (Human), Q5VYE7 (Human), Q6NVY8 (Human)

Additional Laminin gamma 1 Products

Product Documents for Laminin gamma 1 Antibody (A5)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Laminin gamma 1 Antibody (A5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Extracellular Matrix Molecules

Citations for Laminin gamma 1 Antibody (A5)

Customer Reviews for Laminin gamma 1 Antibody (A5) (1)

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  • Laminin gamma 1 Antibody (A5)
    Name: Xing Fu
    Application: Immunohistochemistry-Frozen
    Sample Tested: Skeletal muscle
    Species: Bovine
    Verified Customer | Posted 06/01/2018
    Bovine skeletal muscle. Laminin was stained with anti-laminin antibody (NBP2-44751) (green). Nuclei were stained with Dapi (blue).
    Bovine frozen skeletal muscle section was fixed in 4% PFA for 5 min and stained with anti-laminin antibody (NBP2-44751) overnight at 4 C and then stained with a goat anti-rat-Alexa Fluor 488 secondary antibody (green) for 1 hour at room temperature. Section was then mounted in a mounting medium containing Dapi (blue).
    Laminin gamma 1 Antibody (A5) NBP2-44751

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