Laminin gamma 1 Antibody (CL3196)

Novus Biologicals | Catalog # NBP3-52084

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG1 Clone # CL3196
View all Laminin gamma 1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from P11047, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Laminin gamma 1 Antibody (CL3196)

Laminin gamma 1 Antibody (CL3196) Immunohistochemistry: Laminin gamma 1 Antibody (CL3196) [NBP3-52084]

Immunohistochemistry: Laminin gamma 1 Antibody (CL3196) [NBP3-52084]

Staining of human placenta shows strong positivity in basement membrane of trophoblastic cells.
Laminin gamma 1 Antibody (CL3196) Immunohistochemistry: Laminin gamma 1 Antibody (CL3196) [NBP3-52084]

Immunohistochemistry: Laminin gamma 1 Antibody (CL3196) [NBP3-52084]

Staining of human endometrium shows strong positivity in basement membrane of glandular cells.
Laminin gamma 1 Antibody (CL3196) Western Blot: Laminin gamma 1 Antibody (CL3196) [NBP3-52084]

Western Blot: Laminin gamma 1 Antibody (CL3196) [NBP3-52084]

Analysis of purified human recombinant Laminin-111, Laminin-121, Laminin-211, protein Laminin-221, Laminin-411, Laminin-421, Laminin-511, Laminin-521 and Laminin-332.
Laminin gamma 1 Antibody (CL3196) Western Blot: Laminin gamma 1 Antibody (CL3196) [NBP3-52084]

Western Blot: Laminin gamma 1 Antibody (CL3196) [NBP3-52084]

Analysis in human lung tissue.
Laminin gamma 1 Antibody (CL3196) Immunohistochemistry: Laminin gamma 1 Antibody (CL3196) [NBP3-52084]

Immunohistochemistry: Laminin gamma 1 Antibody (CL3196) [NBP3-52084]

Analysis in human endometrium and tonsil tissues. Corresponding Laminin gamma 1 RNA-seq data are presented for the same tissues.
Laminin gamma 1 Antibody (CL3196) Immunohistochemistry: Laminin gamma 1 Antibody (CL3196) [NBP3-52084]

Immunohistochemistry: Laminin gamma 1 Antibody (CL3196) [NBP3-52084]

Staining of human stomach shows strong positivity in basement membrane of glandular cells.
Laminin gamma 1 Antibody (CL3196) Immunohistochemistry: Laminin gamma 1 Antibody (CL3196) [NBP3-52084]

Immunohistochemistry: Laminin gamma 1 Antibody (CL3196) [NBP3-52084]

Staining of human tonsil shows no positivity in germinal center cells as expected.
Laminin gamma 1 Antibody (CL3196) Immunohistochemistry: Laminin gamma 1 Antibody (CL3196) [NBP3-52084]

Immunohistochemistry: Laminin gamma 1 Antibody (CL3196) [NBP3-52084]

Staining of human heart muscle shows strong positivity in basement membrane of cardiomyocytes.

Applications for Laminin gamma 1 Antibody (CL3196)

Application
Recommended Usage

Immunohistochemistry

1:50-1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, pH 7.2, 40% glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Laminin gamma 1

Laminins are essential and abundant structural non-collagenous glycoproteins localizing to basement membranes. Basement membranes (cell-associated extracellular matrices (ECMs)) are polymers of Laminins with stabilizing Type IV Collagen networks, Nidogen and several proteoglycans. Basement membranes are found under epithelial layers, around the endothelium of blood vessels, and surrounding muscle, peripheral nerve and fat cells. Formation of basement membranes influences cell proliferation, phenotype, migration, gene expression and tissue architecture. Each Laminin is a heterotrimer of alpha, beta and gamma chain subunits that undergoes cell-secretion and incorporation into the ECM. Laminins can self-assemble, bind to other matrix macromolecules and have unique and shared cell interactions mediated by Integrins, dystroglycan and cognate Laminin receptors. The human Laminin gamma-1 gene maps to chromosome 1q31 and is ubiquitously expressed in tissues that produce basement membranes.

Alternate Names

LAMC1, Laminin B2

Gene Symbol

LAMC1

Additional Laminin gamma 1 Products

Product Documents for Laminin gamma 1 Antibody (CL3196)

Certificate of Analysis

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Product Specific Notices for Laminin gamma 1 Antibody (CL3196)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Extracellular Matrix Molecules

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Protocols

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