LEDGF Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-87885

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all LEDGF Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: KQVETEEAGVVTTATASVNLKVSPKRGRPAATEVKIPKPRGRPKMVKQPCPSESDIITEEDKSKKKGQEEKQPKKQPKKDEEGQ

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (81%), Rat (83%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for LEDGF Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: LEDGF Antibody [NBP1-87885]

Immunocytochemistry/ Immunofluorescence: LEDGF Antibody [NBP1-87885]

Immunocytochemistry/Immunofluorescence: LEDGF Antibody [NBP1-87885] - Staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.
LEDGF Antibody - BSA Free Immunohistochemistry-Paraffin: LEDGF Antibody - BSA Free [NBP1-87885]

Immunohistochemistry-Paraffin: LEDGF Antibody - BSA Free [NBP1-87885]

Staining of human kidney shows strong nuclear positivity in cells in tubules.
LEDGF Antibody - BSA Free Immunohistochemistry-Paraffin: LEDGF Antibody - BSA Free [NBP1-87885]

Immunohistochemistry-Paraffin: LEDGF Antibody - BSA Free [NBP1-87885]

Staining of human tonsil shows strong nuclear positivity in non-germinal center cells.
LEDGF Antibody - BSA Free Immunohistochemistry-Paraffin: LEDGF Antibody - BSA Free [NBP1-87885]

Immunohistochemistry-Paraffin: LEDGF Antibody - BSA Free [NBP1-87885]

Staining of human cerebral cortex shows strong nuclear positivity in neuronal cells.
LEDGF Antibody - BSA Free Immunohistochemistry-Paraffin: LEDGF Antibody - BSA Free [NBP1-87885]

Immunohistochemistry-Paraffin: LEDGF Antibody - BSA Free [NBP1-87885]

Staining of human prostate shows strong nuclear positivity in glandular cells.
LEDGF Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: LEDGF Antibody - BSA Free [NBP1-87885]

Chromatin Immunoprecipitation-exo-Seq: LEDGF Antibody - BSA Free [NBP1-87885]

ChIP-Exo-Seq composite graph for Anti-PSIP1 (NBP1-87885) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Applications for LEDGF Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: LEDGF

The human p75 protein is a non TAF transcription coactivator that mediates activator dependent transcription by RNA polymerase II in vitro through most tested activators. Although p75 and p52 are derived from alternatively splicing of a single gene and share most coding sequence, they reveal different function in several aspects. In addition to functioning as a transcription coactivator, p75 has been reported to be involved in regulating growth of epithelial cells, pathogenesis of atopic dermatitis and activity of HIV1 integrase.

Long Name

Lens Epithelium-derived Growth Factor

Alternate Names

CLL-associated antigen KW-7, DFS 70, PSIP1, Transcriptional coactivator p75/p52

Gene Symbol

PSIP1

Additional LEDGF Products

Product Documents for LEDGF Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for LEDGF Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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