LEF1 Antibody (13B7) - BSA Free

Novus Biologicals | Catalog # NBP3-26171

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, ELISA

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 13B7 expressed in HEK293

Format

BSA Free
View all LEF1 Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from Human LEF1 [UniProt Q9UJU2]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for LEF1 Antibody (13B7) - BSA Free

LEF1 Antibody (13B7)

Immunohistochemistry: LEF1 Antibody (13B7) [NBP3-26171] -

Immunohistochemistry: LEF1 Antibody (13B7) [NBP3-26171] - Image of LEF1 Antibody (13B7) diluted at 1:50 and staining in paraffin-embedded human tonsil tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.52% DAB.
LEF1 Antibody (13B7)

Immunohistochemistry: LEF1 Antibody (13B7) [NBP3-26171] -

Immunohistochemistry: LEF1 Antibody (13B7) [NBP3-26171] - Image of LEF1 Antibody (13B7) diluted at 1:50 and staining in paraffin-embedded human tonsil tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.52% DAB.

Applications for LEF1 Antibody (13B7) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50-1:200

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, and 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: LEF1

Lymphoid enhancer-binding factor-1 (LEF1) is a 48-kD nuclear protein that is expressed in pre-B and T cells. It binds to a functionally important site in the T-cell receptor-alpha (TCRA; MIM 186880) enhancer and confers maximal enhancer activity. LEF1 belongs to a family of regulatory proteins that share homology with high mobility group protein-1 (HMG1; MIM 163905).[supplied by OMIM]

Long Name

Lymphoid Enhancer-binding Factor 1

Alternate Names

TCF10, TCF1ALPHA, TCF7L3

Gene Symbol

LEF1

Additional LEF1 Products

Product Documents for LEF1 Antibody (13B7) - BSA Free

Certificate of Analysis

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Product Specific Notices for LEF1 Antibody (13B7) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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