Lgr5/GPR49 Antibody (9J9B8)
Novus Biologicals | Catalog # NBP3-15445
Recombinant Monoclonal Antibody
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Key Product Details
Species Reactivity
Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 9J9B8 expressed in HEK293
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Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 800-907 of human Lgr5/GPR49 (O75473). VIKFILLVVVPLPACLNPLLYILFNPHFKEDLVSLRKQTYVWTRSKHPSLMSINSDDVEKQSCDSTQALVTFTSSSITYDLPPSSVPSPAYPVTESCHLSSVAFVPCL
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Lgr5/GPR49 Antibody (9J9B8)
Western Blot: Lgr5/GPR49 Antibody (9J9B8) [NBP3-15445]
Western Blot: Lgr5/GPR49 Antibody (9J9B8) [NBP3-15445] - Western blot analysis of extracts of various cell lines, using Lgr5/GPR49 Rabbit mAb (NBP3-15445) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.Applications for Lgr5/GPR49 Antibody (9J9B8)
Application
Recommended Usage
Western Blot
1:500 - 1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Lgr5/GPR49
Long Name
Leucine-rich Repeat Containing G Protein-coupled Receptor 5
Alternate Names
FEX, GPR49, GPR67, HG38
Gene Symbol
LGR5
Additional Lgr5/GPR49 Products
Product Documents for Lgr5/GPR49 Antibody (9J9B8)
Certificate of Analysis
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Product Specific Notices for Lgr5/GPR49 Antibody (9J9B8)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars