LIF Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-85717

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Rat (91%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all LIF Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: MKVLAAGVVPLLLVLHWKHGAGSPLPITPVNATCAIRHPCHNNLMNQIRSQLAQLNGSANALFILYYTAQGEPFPNNLDKLCGPNVTDFPPFHANGTEK

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (88%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for LIF Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: LIF Antibody [NBP1-85717]

Immunocytochemistry/ Immunofluorescence: LIF Antibody [NBP1-85717]

Immunocytochemistry/Immunofluorescence: LIF Antibody [NBP1-85717] - Staining of human cell line A-431 shows localization to cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: LIF Antibody [NBP1-85717]

Immunohistochemistry-Paraffin: LIF Antibody [NBP1-85717]

Immunohistochemistry-Paraffin: LIF Antibody [NBP1-85717] - Staining of human endometrium shows strong positivity in cilia.
Immunohistochemistry-Paraffin: LIF Antibody [NBP1-85717]

Immunohistochemistry-Paraffin: LIF Antibody [NBP1-85717]

Immunohistochemistry-Paraffin: LIF Antibody [NBP1-85717] - Staining of human cerebral cortex shows moderate positivity.
Immunohistochemistry-Paraffin: LIF Antibody [NBP1-85717]

Immunohistochemistry-Paraffin: LIF Antibody [NBP1-85717]

Immunohistochemistry-Paraffin: LIF Antibody [NBP1-85717] - Staining of human lung shows strong cytoplasmic positivity.
Immunohistochemistry-Paraffin: LIF Antibody [NBP1-85717]

Immunohistochemistry-Paraffin: LIF Antibody [NBP1-85717]

Immunohistochemistry-Paraffin: LIF Antibody [NBP1-85717] - Staining of human prostate cancer shows moderate cytoplasmic positivity.
Immunohistochemistry-Paraffin: LIF Antibody [NBP1-85717]

Immunohistochemistry-Paraffin: LIF Antibody [NBP1-85717]

Immunohistochemistry-Paraffin: LIF Antibody [NBP1-85717] - Staining of human urinary bladder shows moderate cytoplasmic positivity.
LIF Antibody - BSA Free Western Blot: LIF Antibody - BSA Free [NBP1-85717]

Western Blot: LIF Antibody - BSA Free [NBP1-85717]

Analysis in human cell lines U-251MG and HEK293 using Anti-LIF antibody. Corresponding LIF RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.

Applications for LIF Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: LIF

LIF (leukemia inhibitory factor, myeloid leukemia inhibitory factor) a pleiotropic lymphoid factor was initially described as a factor that inhibits the proliferation of myeloid leukemia cells and induces their differentiation into macrophages. Other activities of LIF include inhibition of adipogenesis, cholinergic neuron differentiation and bone metabolism. Human and mouse LIF share 78% homology and activities of LIF are not species-specific. Human LIF is active on mouse cells, but murine LIF is not active on human cells. Benefits of NovActive® Proteins
Fully Active
-Fully folded naturally by eukaryotic source
-Comparable for eukaryotic glycosylation
BioRisk Free
-animal-free
-serum-free
-endotoxin-free
-infection reagent-free
-low in proteolyic activity
-low in pyrogenic and pro-inflammatory activity
Just reconstitute and add to your cell culture.
Have FDA G.R.A.S. (generally recognized as safe) approval.
Advantages of barley endosperm Folding -Proficient protein machinery, with eukaryotic folding, allows for native formation of the protein and long term protection and storage. Environment -A biochemically inert environment, void of endotoxins, low protease activity and secondary metabolite content, and a simple protein profile, aid in downstream processing in barley. FDA G.R.A.S status -Barley is recognized by the FDA as having G.R.A.S (generally recognized as safe) status.

Long Name

Leukemia Inhibitory Factor

Alternate Names

D Factor, Emfilermin, HILDA, MLPLI

Gene Symbol

LIF

Additional LIF Products

Product Documents for LIF Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for LIF Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for LIF Antibody - BSA Free

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Protocols

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FAQs

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