Recombinant Bovine LIF Protein

Name: Recombinant Bovine LIF Protein
Brand: Novus Biologicals
SKU: NBP2-62483
Price: 349 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-62483

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Product Specifications
Scientific Data
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Source

Pichia pastoris

Accession Number

Q27956

Applications

Western Blot, ELISA

View all LIF Proteins and Enzymes »

Product Specifications

Description

Recombinant bovine leukemia inhibitory factor.

Source:Pichia pastoris

Amino Acid Sequence:aa 23-202 of native bovine LIF, UniProt Q27956

Purity

>95%, by SDS-PAGE

Predicted Molecular Mass

19.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Bovine LIF Protein

ELISA: Recombinant Bovine LIF Protein [NBP2-62483] -

ELISA: Recombinant Bovine LIF Protein [NBP2-62483] - Bovine Leukemia Inhibitory Factor ELISA

Recombinant Bovine leukemia inhibitory factor detected using Chicken anti Bovine leukemia inhibitory factor as the capture reagent and Chicken anti Bovine leukemia inhibitory factor:Biotin as the detection reagent followed by Streptavidin:HRP

Formulation, Preparation, and Storage

NBP2-62483

Preparation Method	Recombinant protein expressed in Pichia pastoris and purified by ion-exchange chromatography.
Formulation	PBS
Preservative	10% Trehalose
Concentration	Lyoph
Reconstitution	Reconstitute with 20 ul sterile PBS containing 0.1% carrier protein. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Gently mix vial after reconstitution.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: LIF

LIF (leukemia inhibitory factor, myeloid leukemia inhibitory factor) a pleiotropic lymphoid factor was initially described as a factor that inhibits the proliferation of myeloid leukemia cells and induces their differentiation into macrophages. Other activities of LIF include inhibition of adipogenesis, cholinergic neuron differentiation and bone metabolism. Human and mouse LIF share 78% homology and activities of LIF are not species-specific. Human LIF is active on mouse cells, but murine LIF is not active on human cells. Benefits of NovActive® Proteins

Fully Active: -Fully folded naturally by eukaryotic source; -Comparable for eukaryotic glycosylation
BioRisk Free: -animal-free; -serum-free; -endotoxin-free; -infection reagent-free; -low in proteolyic activity; -low in pyrogenic and pro-inflammatory activity
Just reconstitute and add to your cell culture.
Have FDA G.R.A.S. (generally recognized as safe) approval.

Advantages of barley endosperm Folding -Proficient protein machinery, with eukaryotic folding, allows for native formation of the protein and long term protection and storage. Environment -A biochemically inert environment, void of endotoxins, low protease activity and secondary metabolite content, and a simple protein profile, aid in downstream processing in barley. FDA G.R.A.S status -Barley is recognized by the FDA as having G.R.A.S (generally recognized as safe) status.

Long Name

Leukemia Inhibitory Factor

Alternate Names

D Factor, Emfilermin, HILDA, MLPLI

Gene Symbol

LIF

UniProt

Q27956 (Bovine)

Additional LIF Products

Product Documents for Recombinant Bovine LIF Protein

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Certificate of Analysis

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Product Specific Notices for Recombinant Bovine LIF Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

Related Research Areas

Cytokines and Receptors in Allergy and Asthma

Diabetes

IL-6 Family

Obesity

Reproduction

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