LIM Kinase 1 Antibody (OTI3H6)

Novus Biologicals | Catalog # NBP2-00748

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Monkey

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI3H6
View all LIM Kinase 1 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human LIMK1(NP_002305) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

72.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for LIM Kinase 1 Antibody (OTI3H6)

Western Blot: LIM Kinase 1 Antibody (3H6) [NBP2-00748] Analysis of extracts (35ug) from 9 different cell lines by using anti-LIM Kinase 1 monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: LIM Kinase 1 Antibody (OTI3H6) [NBP2-00748]

Immunocytochemistry/ Immunofluorescence: LIM Kinase 1 Antibody (OTI3H6) [NBP2-00748]

Immunocytochemistry/Immunofluorescence: LIM Kinase 1 Antibody (3H6) [NBP2-00748] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY LIM Kinase 1.
Immunohistochemistry-Paraffin: LIM Kinase 1 Antibody (OTI3H6) [NBP2-00748]

Immunohistochemistry-Paraffin: LIM Kinase 1 Antibody (OTI3H6) [NBP2-00748]

Immunohistochemistry-Paraffin: LIM Kinase 1 Antibody (3H6) [NBP2-00748] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-LIM Kinase 1 mouse monoclonal antibody.
Flow Cytometry: LIM Kinase 1 Antibody (OTI3H6) [NBP2-00748]

Flow Cytometry: LIM Kinase 1 Antibody (OTI3H6) [NBP2-00748]

Flow Cytometry: LIM Kinase 1 Antibody (3H6) [NBP2-00748] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-LIM Kinase 1 antibody, and then analyzed by flow cytometry.
Western Blot: LIM Kinase 1 Antibody (OTI3H6) [NBP2-00748]

Western Blot: LIM Kinase 1 Antibody (OTI3H6) [NBP2-00748]

Western Blot: LIM Kinase 1 Antibody (3H6) [NBP2-00748] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LIM Kinase 1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LIM Kinase 1.
Immunohistochemistry-Paraffin: LIM Kinase 1 Antibody (OTI3H6) [NBP2-00748]

Immunohistochemistry-Paraffin: LIM Kinase 1 Antibody (OTI3H6) [NBP2-00748]

Immunohistochemistry-Paraffin: LIM Kinase 1 Antibody (3H6) [NBP2-00748] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-LIM Kinase 1 mouse monoclonal antibody.

Applications for LIM Kinase 1 Antibody (OTI3H6)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:500-2000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: LIM Kinase 1

There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. LIMK1 is a serine/threonine kinase that regulates actin polymerization via phosphorylation and inactivation of the actin binding factor cofilin. This protein is ubiquitously expressed during development and plays a role in many cellular processes associated with cytoskeletal structure. This protein also stimulates axon growth and may play a role in brain development. LIMK1 hemizygosity is implicated in the impaired visuospatial constructive cognition of Williams syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Long Name

LIM domain kinase 1

Alternate Names

LIMK, LIMK-1, LIMK1

Entrez Gene IDs

3984 (Human)

Gene Symbol

LIMK1

Additional LIM Kinase 1 Products

Product Documents for LIM Kinase 1 Antibody (OTI3H6)

Certificate of Analysis

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Product Specific Notices for LIM Kinase 1 Antibody (OTI3H6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for LIM Kinase 1 Antibody (OTI3H6)

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Protocols

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