LIM kinase 2 Antibody

Novus Biologicals | Catalog # NBP3-12321

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Chicken
View all LIM kinase 2 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide taken within amino acid region 570-640 on LIMK2 protein.

Clonality

Polyclonal

Host

Chicken

Scientific Data Images for LIM kinase 2 Antibody

Immunohistochemistry-Paraffin: LIM kinase 2 Antibody [NBP3-12321]

Immunohistochemistry-Paraffin: LIM kinase 2 Antibody [NBP3-12321]

Immunohistochemistry-Paraffin: LIM kinase 2 Antibody [NBP3-12321] - THP cells. 1:500 antibody in DiluObuffer. Apparent MW is 76 KDa.
Immunohistochemistry-Paraffin: LIM kinase 2 Antibody [NBP3-12321]

Immunohistochemistry-Paraffin: LIM kinase 2 Antibody [NBP3-12321]

Immunohistochemistry-Paraffin: LIM kinase 2 Antibody [NBP3-12321] - Mouse Liver. 1:50 dilution in IHC blocking buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstain. 40X magnification.

Applications for LIM kinase 2 Antibody

Application
Recommended Usage

ELISA

1:10000

Immunohistochemistry

1:50-1:150

Immunohistochemistry-Paraffin

1:50-1:150

Immunoprecipitation

1:200

Western Blot

1:500

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

Contains Tris, HCl/glycine buffer (pH 7.4-7.8), 30% glycerol, 0.5% BSA, along with cryo-protective agents, and HEPES (exact storage and stabilization buffer information is proprietary).

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Store at -20C long term. Avoid freeze-thaw cycles.

Background: LIM kinase 2

LIM-kinase 2 (LIMK2), a 75-kDa member of the LIM kinase family, is responsible for regulating actin polymerization via phosphorylation and inactivation of the actin depolymerizing factors ADF/cofilin (1). LIMK2 protein is found in the cytoplasm during interphase, redistributed to the mitotic spindle and then to the spindle midzone during anaphase to telophase (2). It has been shown that ROCK, a downstream protein kinase of Rho, activates LIMK2 by directly phosphorylates threonine 505 residue. Since activity of LIMK2 is cell cycle dependant, LIMK2 activity is markedly increased when HeLa cells are synchronized at mitosis with nocodazole treatment (3).

Long Name

LIM domain kinase 2

Alternate Names

LIMK-2, LIMK2

Gene Symbol

LIMK2

Additional LIM kinase 2 Products

Product Documents for LIM kinase 2 Antibody

Certificate of Analysis

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Product Specific Notices for LIM kinase 2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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