Lipin 2 Antibody

Novus Biologicals | Catalog # NBP3-00525

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Peptide ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Lipin 2 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence CYWRDPIPEVDLDDLS corresponding to C-Terminus according to NP_055461.1.

Epitope

CYWRDPIPEVDLDDLS

Reactivity Notes

Expected from sequence similarity: Mouse, Rat, Porcine

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Lipin 2 Antibody

Immunocytochemistry/ Immunofluorescence: Lipin 2 Antibody [NBP3-00525]

Immunocytochemistry/ Immunofluorescence: Lipin 2 Antibody [NBP3-00525]

Immunocytochemistry/Immunofluorescence: Lipin 2 Antibody [NBP3-00525] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor (TM) 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor (TM) 488 secondary antibody (2ug/ml).
Flow Cytometry: Lipin 2 Antibody [NBP3-00525]

Flow Cytometry: Lipin 2 Antibody [NBP3-00525]

Flow Cytometry: Lipin 2 Antibody [NBP3-00525] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor (TM) 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor (TM) 488 secondary antibody.
Immunocytochemistry/ Immunofluorescence: Lipin 2 Antibody [NBP3-00525]

Immunocytochemistry/ Immunofluorescence: Lipin 2 Antibody [NBP3-00525]

Immunocytochemistry/Immunofluorescence: Lipin 2 Antibody [NBP3-00525] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor (TM) 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor (TM) 488 secondary antibody (2ug/ml).

Applications for Lipin 2 Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/mL

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Peptide ELISA

Detection limit 1:8000
Application Notes
Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A431 and U2OS cells.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Lipin 2

Lipin 2 plays important roles in controlling the metabolism of fatty acids at differents levels. Lipin 2 acts as a magnesium-dependent phosphatidate phosphatase enzyme which catalyzes the conversion of phosphatidic acid to diacylglycerol during triglyceride, phosphatidylcholine and phosphatidylethanolamine biosynthesis in the reticulum endoplasmic membrane. Lipin 2 also acts also as a nuclear transcriptional coactivator for PPARGC1A to modulate lipid metabolism By similarity. Defects in LPIN2 are the cause of Majeed syndrome (MAJEEDS) [MIM:609628]. An autosomal recessive syndrome characterized by chronic recurrent multifocal osteomyelitis that is of early onset with a lifelong course, congenital dyserythropoietic anemia that presents as hypochromic, microcytic anemia during the first year of life and ranges from mild to transfusion-dependent, and transient inflammatory dermatosis, often manifesting as Sweet syndrome (neutrophilic skin infiltration).

Long Name

Phosphatidate Phosphatase LPIN2

Alternate Names

LPIN2

Gene Symbol

LPIN2

Additional Lipin 2 Products

Product Documents for Lipin 2 Antibody

Certificate of Analysis

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Product Specific Notices for Lipin 2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Obesity
Phosphatases and Regulators

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Protocols

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