Lipocalin-2/NGAL Antibody (14) - BSA Free

Novus Biologicals | Catalog # NBP2-41364

Novus Biologicals
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Key Product Details

Species Reactivity

Canine, Human (Negative), Mouse (Negative), Rat (Negative)

Applications

Immunohistochemistry, Western Blot, ELISA, Sandwich ELISA, Sandwich ELISA Detection

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 14

Format

BSA Free
View all Lipocalin-2/NGAL Primary Antibodies »

Product Specifications

Immunogen

Recombinant dog NGAL

Epitope

Not determined

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for Lipocalin-2/NGAL Antibody (14) - BSA Free

Immunohistochemistry: Lipocalin-2/NGAL Antibody (14) - BSA Free [NBP2-41364]
Sandwich ELISA: Lipocalin-2/NGAL Antibody (14) - BSA Free [NBP2-41364] - Analysis using the Biotin conjugate of NBP2-41364. The calibration curveusing NBP2-23595 as the biotinylated detection antibody and NBP2-41364 as the capture antibody.

Applications for Lipocalin-2/NGAL Antibody (14) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunohistochemistry

1:10-1:500

Western Blot

1:100-1:2000
Application Notes
ELISA: NBP2-41364 binds free dog NGAL. NBP2-41364 (as biotinylated detection antibody) forms a sandwich ELISA pair with NBP2-23599 (as capture antibody) for measuring dog NGAL, giving a detection limit of 5 pg/mL in an unoptimized buffer assay. WB: NBP2-41364 can be used in Western blotting. IHC: NBP2-41364 was used in IHC in formaline fixed paraffin embedded tissue with a dilution of 1:50.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM Phosphate (pH 7.4) and 0.5M NaCl

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Lipocalin-2/NGAL

Lipocalin 2 is a member of the lipocalin family which encompass more than 25 members (including proteins like retinol binding protein, a1 microglobulin, b lactoglobulin, apolipoprotein D, odorant binding protein). The members of the lipocalin family are characterized by their ability to bind small lipophilic substances in their hydrophobic core, and thereby serve as transporters of substances.

Long Name

Neutrophil Gelatinase-associated Lipocalin

Alternate Names

24p3, LCN2, Lipocalin2, MSFI, NGAL, Oncogene 24p3, p25, Siderocalin, Uterocalin

Gene Symbol

LCN2

Additional Lipocalin-2/NGAL Products

Product Documents for Lipocalin-2/NGAL Antibody (14) - BSA Free

Certificate of Analysis

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Product Specific Notices for Lipocalin-2/NGAL Antibody (14) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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