LMAN1 Antibody (3A1X6)

Novus Biologicals | Catalog # NBP3-16517

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 3A1X6 expressed in HEK293
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Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human LMAN1 (P49257). MAGSRQRGLRARVRPLFCALLLSLGRFVRGDGVGGDPAVALPHRRFEYKYSFKGPHLVQSDGTVPFWAHAGNAIPSSDQIRVAPSLKSQRGSVWTKTKAA

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for LMAN1 Antibody (3A1X6)

Western Blot: LMAN1 Antibody (3A1X6) [NBP3-16517]

Western Blot: LMAN1 Antibody (3A1X6) [NBP3-16517]

Western Blot: LMAN1 Antibody (3A1X6) [NBP3-16517] - Western blot analysis of extracts of various cell lines, using LMAN1 Rabbit mAb (NBP3-16517) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry-Paraffin: LMAN1 Antibody (3A1X6) [NBP3-16517]

Immunohistochemistry-Paraffin: LMAN1 Antibody (3A1X6) [NBP3-16517]

Immunohistochemistry-Paraffin: LMAN1 Antibody (3A1X6) [NBP3-16517] - Immunohistochemistry of paraffin-embedded human placenta using LMAN1 Rabbit mAb (NBP3-16517) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: LMAN1 Antibody (3A1X6) [NBP3-16517]

Immunohistochemistry-Paraffin: LMAN1 Antibody (3A1X6) [NBP3-16517]

Immunohistochemistry-Paraffin: LMAN1 Antibody (3A1X6) [NBP3-16517] - Immunohistochemistry of paraffin-embedded rat brain using LMAN1 Rabbit mAb (NBP3-16517) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: LMAN1 Antibody (3A1X6) [NBP3-16517]

Immunohistochemistry-Paraffin: LMAN1 Antibody (3A1X6) [NBP3-16517]

Immunohistochemistry-Paraffin: LMAN1 Antibody (3A1X6) [NBP3-16517] - Immunohistochemistry of paraffin-embedded human appendix using LMAN1 Rabbit mAb (NBP3-16517) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Applications for LMAN1 Antibody (3A1X6)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: LMAN1

Protein trafficking along the endoplasmic reticulum (ER) to the Golgi apparatus occurs by vesicular transport between membranous compartments. LMAN1 is a mannose-specific lectin transport receptor protein which cycles between the ER and the Golgi complex. LMAN1 is involved in intracellular transport of many glycoproteins. LMAN1 is useful in studying mechanisms underlying protein trafficking early in the secretory pathway.

Alternate Names

ERGIC-53, ERGIC53ER-Golgi intermediate compartment 53 kDa protein, F5F8Dendoplasmic reticulum-golgi intermediate compartment protein 53, FMFD1, Gp58, intracellular mannose specific lectin, Intracellular mannose-specific lectin MR60, Lectin mannose-binding 1, lectin, mannose-binding, 1, MCFD1coagulation factor V-factor VIII combined deficiency, MR60, protein ERGIC-53

Gene Symbol

LMAN1

Additional LMAN1 Products

Product Documents for LMAN1 Antibody (3A1X6)

Certificate of Analysis

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Product Specific Notices for LMAN1 Antibody (3A1X6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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