LMO2 Antibody (LMO2/1971)
Novus Biologicals | Catalog # NBP3-07249
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # LMO2/1971
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Product Specifications
Immunogen
Recombinant human LMO2 protein fragment (around aa 23-140) (exact sequence is proprietary) (Uniprot: P25791)
Reactivity Notes
Others not known.
Localization
Nuclear, Cytoplasmic & Cell Surface
Marker
B-Cell Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Theoretical MW
24 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08268)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for LMO2 Antibody (LMO2/1971)
![Western Blot: LMO2 Antibody (LMO2/1971) [NBP3-07249]](https://resources.rndsystems.com/images/products/LMO2-Antibody-LMO2-1971-Western-Blot-NBP3-07249-img0004.jpg "Western Blot: LMO2 Antibody (LMO2/1971) [NBP3-07249]")
Western Blot: LMO2 Antibody (LMO2/1971) [NBP3-07249]
Western Blot: LMO2 Antibody (LMO2/1971) [NBP3-07249] - Western Blot Analysis of Ramos cell lysate using LMO2 Mouse Monoclonal Antibody (LMO2/1971).![Immunohistochemistry-Paraffin: LMO2 Antibody (LMO2/1971) [NBP3-07249]](https://resources.rndsystems.com/images/products/LMO2-Antibody-LMO2-1971-Immunohistochemistry-Paraffin-NBP3-07249-img0003.jpg "Immunohistochemistry-Paraffin: LMO2 Antibody (LMO2/1971) [NBP3-07249]")
Immunohistochemistry-Paraffin: LMO2 Antibody (LMO2/1971) [NBP3-07249]
Immunohistochemistry-Paraffin: LMO2 Antibody (LMO2/1971) [NBP3-07249] - Formalin-fixed, paraffin-embedded human Pancreas stained with LMO2 Mouse Monoclonal Antibody (LMO2/1971).![Flow Cytometry: LMO2 Antibody (LMO2/1971) [NBP3-07249]](https://resources.rndsystems.com/images/products/LMO2-Antibody-LMO2-1971-Flow-Cytometry-NBP3-07249-img0001.jpg "Flow Cytometry: LMO2 Antibody (LMO2/1971) [NBP3-07249]")
Flow Cytometry: LMO2 Antibody (LMO2/1971) [NBP3-07249]
Flow Cytometry: LMO2 Antibody (LMO2/1971) [NBP3-07249] - Flow Cytometric Analysis of K562 cells using LMO2 Mouse Monoclonal Antibody (LMO2/1971) followed by goat anti- Mouse IgG-CF488 (Blue); Isotype Control (Red)![Immunohistochemistry-Paraffin: LMO2 Antibody (LMO2/1971) [NBP3-07249]](https://resources.rndsystems.com/images/products/LMO2-Antibody-LMO2-1971-Immunohistochemistry-Paraffin-NBP3-07249-img0002.jpg "Immunohistochemistry-Paraffin: LMO2 Antibody (LMO2/1971) [NBP3-07249]")
Immunohistochemistry-Paraffin: LMO2 Antibody (LMO2/1971) [NBP3-07249]
Immunohistochemistry-Paraffin: LMO2 Antibody (LMO2/1971) [NBP3-07249] - Formalin-fixed, paraffin-embedded human Follicular Lymphoma stained with LMO2 Mouse Monoclonal Antibody (LMO2/1971).Applications for LMO2 Antibody (LMO2/1971)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunohistochemistry
1-2 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Western Blot
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: LMO2
LMO2 antibodies are useful tools for erythropoiesis research and certian cancer studies.
Long Name
LIM Domain Only 2
Alternate Names
RBTN2, RBTNL1, RHOM2, TTG2
Gene Symbol
LMO2
UniProt
Additional LMO2 Products
Product Documents for LMO2 Antibody (LMO2/1971)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for LMO2 Antibody (LMO2/1971)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
