LMP2/PSMB9 Antibody (OTI1D1)

Novus Biologicals | Catalog # NBP2-01817

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI1D1
View all LMP2/PSMB9 Primary Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 21-219 of human PSMB9(NP_002791) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

23.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for LMP2/PSMB9 Antibody (OTI1D1)

Western Blot: LMP2/PSMB9 Antibody (OTI1D1) [NBP2-01817]

Western Blot: LMP2/PSMB9 Antibody (OTI1D1) [NBP2-01817]

Western Blot: LMP2/PSMB9 Antibody (OTI1D1) [NBP2-01817] - Analysis of extracts (10ug) from Jurkat cell line by using anti-PSMB9 monoclonal antibody (1:200).
Immunocytochemistry/ Immunofluorescence: LMP2/PSMB9 Antibody (OTI1D1) [NBP2-01817]

Immunocytochemistry/ Immunofluorescence: LMP2/PSMB9 Antibody (OTI1D1) [NBP2-01817]

Immunocytochemistry/Immunofluorescence: LMP2/PSMB9 Antibody (OTI1D1) [NBP2-01817] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Proteasome 20S LMP2.
Immunohistochemistry-Paraffin: LMP2/PSMB9 Antibody (OTI1D1) [NBP2-01817]

Immunohistochemistry-Paraffin: LMP2/PSMB9 Antibody (OTI1D1) [NBP2-01817]

Immunohistochemistry-Paraffin: LMP2/PSMB9 Antibody (1D1) [NBP2-01817] - Human tonsil.
Flow Cytometry: LMP2/PSMB9 Antibody (OTI1D1) [NBP2-01817]

Flow Cytometry: LMP2/PSMB9 Antibody (OTI1D1) [NBP2-01817]

Flow Cytometry: LMP2/PSMB9 Antibody (1D1) [NBP2-01817] - Analysis of Jurkat cells, using anti-Proteasome 20S LMP2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Western Blot: LMP2/PSMB9 Antibody (OTI1D1) [NBP2-01817]

Western Blot: LMP2/PSMB9 Antibody (OTI1D1) [NBP2-01817]

Western Blot: LMP2/PSMB9 Antibody (1D1) [NBP2-01817] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Proteasome 20S LMP2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Proteasome 20S LMP2.
Immunohistochemistry-Paraffin: LMP2/PSMB9 Antibody (OTI1D1) [NBP2-01817]

Immunohistochemistry-Paraffin: LMP2/PSMB9 Antibody (OTI1D1) [NBP2-01817]

Immunohistochemistry-Paraffin: LMP2/PSMB9 Antibody (1D1) [NBP2-01817] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Proteasome 20S LMP2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: LMP2/PSMB9 Antibody (OTI1D1) [NBP2-01817]

Immunohistochemistry-Paraffin: LMP2/PSMB9 Antibody (OTI1D1) [NBP2-01817]

Immunohistochemistry-Paraffin: LMP2/PSMB9 Antibody (1D1) [NBP2-01817] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-Proteasome 20S LMP2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: LMP2/PSMB9 Antibody (OTI1D1) [NBP2-01817]

Immunohistochemistry-Paraffin: LMP2/PSMB9 Antibody (OTI1D1) [NBP2-01817]

Immunohistochemistry-Paraffin: LMP2/PSMB9 Antibody (1D1) [NBP2-01817] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-Proteasome 20S LMP2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: LMP2/PSMB9 Antibody (OTI1D1) [NBP2-01817]

Immunohistochemistry-Paraffin: LMP2/PSMB9 Antibody (OTI1D1) [NBP2-01817]

Immunohistochemistry-Paraffin: LMP2/PSMB9 Antibody (1D1) [NBP2-01817] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-Proteasome 20S LMP2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: LMP2/PSMB9 Antibody (OTI1D1) [NBP2-01817]

Immunohistochemistry-Paraffin: LMP2/PSMB9 Antibody (OTI1D1) [NBP2-01817]

Immunohistochemistry-Paraffin: LMP2/PSMB9 Antibody (1D1) [NBP2-01817] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-Proteasome 20S LMP2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: LMP2/PSMB9 Antibody (OTI1D1) [NBP2-01817]

Immunohistochemistry-Paraffin: LMP2/PSMB9 Antibody (OTI1D1) [NBP2-01817]

Immunohistochemistry-Paraffin: LMP2/PSMB9 Antibody (1D1) [NBP2-01817] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-Proteasome 20S LMP2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: LMP2/PSMB9 Antibody (OTI1D1) [NBP2-01817]

Immunohistochemistry-Paraffin: LMP2/PSMB9 Antibody (OTI1D1) [NBP2-01817]

Immunohistochemistry-Paraffin: LMP2/PSMB9 Antibody (1D1) [NBP2-01817] - Staining of paraffin-embedded Human Kidney tissue using anti-Proteasome 20S LMP2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: LMP2/PSMB9 Antibody (OTI1D1) [NBP2-01817]

Immunohistochemistry-Paraffin: LMP2/PSMB9 Antibody (OTI1D1) [NBP2-01817]

Immunohistochemistry-Paraffin: LMP2/PSMB9 Antibody (1D1) [NBP2-01817] - Staining of paraffin-embedded Human prostate tissue using anti-Proteasome 20S LMP2 mouse monoclonal antibody.

Applications for LMP2/PSMB9 Antibody (OTI1D1)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:2000

Flow Cytometry Panel Builder

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA, 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1.29 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: LMP2/PSMB9

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is located in the class II region of the MHC (major histocompatibility complex). Expression of this gene is induced by gamma interferon and this gene product replaces catalytic subunit 1 (proteasome beta 6 subunit) in the immunoproteasome. Proteolytic processing is required to generate a mature subunit. [provided by RefSeq].

Long Name

Proteasome (Prosome, Macropain) Subunit, beta Type, 9

Alternate Names

Proteasome subunit beta-1i, PSMB9, RING12

Entrez Gene IDs

5698 (Human)

Gene Symbol

PSMB9

Additional LMP2/PSMB9 Products

Product Documents for LMP2/PSMB9 Antibody (OTI1D1)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for LMP2/PSMB9 Antibody (OTI1D1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

T Cell Antigen Recognition

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Protocols

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