LPAR1/LPA1/EDG-2 Antibody (OTI1G6)

Novus Biologicals | Catalog # NBP2-01354

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Rat

Applications

Validated:

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI1G6
View all LPAR1/LPA1/EDG-2 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human LPAR1(NP_001392) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

40.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for LPAR1/LPA1/EDG-2 Antibody (OTI1G6)

Immunocytochemistry/ Immunofluorescence: LPAR1/LPA1/EDG-2 Antibody (OTI1G6) [NBP2-01354]

Immunocytochemistry/ Immunofluorescence: LPAR1/LPA1/EDG-2 Antibody (OTI1G6) [NBP2-01354]

Immunocytochemistry/Immunofluorescence: LPAR1/LPA1/EDG-2 Antibody (1G6) [NBP2-01354] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY EDG2.
Flow Cytometry: LPAR1/LPA1/EDG-2 Antibody (OTI1G6) [NBP2-01354]

Flow Cytometry: LPAR1/LPA1/EDG-2 Antibody (OTI1G6) [NBP2-01354]

Flow Cytometry: LPAR1/LPA1/EDG-2 Antibody (1G6) [NBP2-01354] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-EDG2 antibody, and then analyzed by flow cytometry.
LPAR1/LPA1/EDG-2 Antibody (OTI1G6)

Western Blot: LPAR1/LPA1/EDG-2 Antibody (1G6) [NBP2-01354] -

Analysis of overexpressedlysates (25ug per lane) from HEK293T cellstransfected with empty plasmid, lane1), human LPAR1 plasmid,lane 2),mouse LPAR1 plasmid ([MR205601], lane 3), ratLPAR1 plasmid, lane 4) using antiLPAR1 antibody (1:500). Figure B,Western blot analysis of the same samples asfigure A with anti-DDK antibody,1:1000)

Applications for LPAR1/LPA1/EDG-2 Antibody (OTI1G6)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:2000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.8 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: LPAR1/LPA1/EDG-2

The integral membrane protein encoded by this gene is a lysophosphatidic acid (LPA) receptor from a group known as EDG receptors. These receptors are members of the G protein-coupled receptor superfamily. Utilized by LPA for cell signaling, EDG receptors mediate diverse biologic functions, including proliferation, platelet aggregation, smooth muscle contraction, inhibition of neuroblastoma cell differentiation, chemotaxis, and tumor cell invasion. Two transcript variants encoding the same protein have been identified for this gene [provided by RefSeq].

Long Name

Lysophosphatidic Acid Receptor 1

Alternate Names

EDG2, GPCR26, GPR26, LPA1, rec.1.3, vzg-1

Entrez Gene IDs

1902 (Human)

Gene Symbol

LPAR1

Additional LPAR1/LPA1/EDG-2 Products

Product Documents for LPAR1/LPA1/EDG-2 Antibody (OTI1G6)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for LPAR1/LPA1/EDG-2 Antibody (OTI1G6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for LPAR1/LPA1/EDG-2 Antibody (OTI1G6)

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Protocols

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FAQs

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