LRP-1 (low-density lipoprotein receptor-related protein 1) is a large type I transmembrane protein belonging to the LDL receptor superfamily. The extracellular domain of LRP-1 is organized in four clusters of ligand-binding repeats that recognizes at least 30 different ligands. The amino acid sequence of human and mouse LRP-1 cluster II share 100% identity.
LRP-1 Antibody - BSA Free
Novus Biologicals | Catalog # NBP2-62753
![Western Blot: LRP-1 Antibody [NBP2-62753]](https://resources.rndsystems.com/images/products/LRP-1-Antibody-Western-Blot-NBP2-62753-img0003.jpg "Western Blot: LRP-1 Antibody [NBP2-62753]")
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
Synthetic peptide from the N-terminal of Human Low-density lipoprotein receptor-related protein 1 (aa. 160-260)
Specificity
Detects 80 kDa. Band at 80 kDa is a membrane spanning subunit.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for LRP-1 Antibody - BSA Free
Western Blot: LRP-1 Antibody [NBP2-62753]
Western Blot: LRP-1 Antibody [NBP2-62753] - Western blot analysis of Mouse, Rat Brain showing detection of 504.6 kDa LRP-1 protein using Rabbit Anti-LRP-1 Polyclonal Antibody (NBP2-62753). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Brain. Lane 3: Rat Brain. Load: 15 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-LRP-1 Polyclonal Antibody (NBP2-62753) at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: 504.6 kDa. Other Band(s): 80 kDa.![Immunohistochemistry: LRP-1 Antibody [NBP2-62753]](https://resources.rndsystems.com/images/products/LRP-1 Antibody-Immunohistochemistry-NBP2-62753-img0004.jpg "Immunohistochemistry: LRP-1 Antibody [NBP2-62753]")
Immunohistochemistry: LRP-1 Antibody [NBP2-62753]
Immunohistochemistry: LRP-1 Antibody [NBP2-62753] - Immunohistochemistry analysis using Rabbit Anti-LRP1 Polyclonal Antibody (NBP2-62753). Tissue: Liver. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-LRP1 Polyclonal Antibody (NBP2-62753) at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection.Applications for LRP-1 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:50
Western Blot
1:1000
Application Notes
A 1:1000 dilution of was sufficient for detection of LRP-1 in 15 ug of mouse Brain cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Formulation, Preparation, and Storage
Purification
Peptide affinity purified
Formulation
PBS (pH 7.4), 50% Glycerol
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: LRP-1
Long Name
LDL Receptor-related Protein 1
Alternate Names
A2MR, Alpha-2-Macroglobulin Receptor, APOER, Apolipoprotein E receptor, APR, CD91, CD91 antigen, EC 1.1.1.94, EC 3.4.21.9, IGFBP3R, low density lipoprotein receptor-related protein 1, LRP, LRP-1, LRP1A, prolow-density lipoprotein receptor-related protein 1, TbetaR-V/LRP-1/IGFBP-3 receptor, TGFBR5, type V tgf-beta receptor
Gene Symbol
LRP1
Additional LRP-1 Products
Product Documents for LRP-1 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for LRP-1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for LRP-1 Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
