Human LRP-6 ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP3-00411

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, cell supernatant, and other biological fluids (100 ul)

Sensitivity

0.19 ng/mL

Assay Range

0.31 - 20 ng/mL
View all LRP-6 ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Human

Specificity

This kit recognizes Human LRP-6 in samples. No significant cross-reactivity or interference between Human LRP-6 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) 8.73%

Inter-Assay Precision (Precision between assays) 10.05%

Recovery for Human LRP-6 ELISA Kit (Colorimetric)

Recovery

87 - 106%

Linearity

Human LRP-6 ELISA Kit (Colorimetric)

Scientific Data Images for Human LRP-6 ELISA Kit (Colorimetric)

Human LRP-6 ELISA Kit (Colorimetric)

ELISA: Human LRP-6 ELISA Kit (Colorimetric) [NBP3-00411] -

ELISA: Human LRP-6 ELISA Kit (Colorimetric) [NBP3-00411] - Standard Curve Reference

Kit Contents for Human LRP-6 ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: LRP-6

Please note, this product is one of a range of Investigative Grade antibodies, made against targets that have limited or no commercial antibodies available to them and for which there are no data on the expression of the protein in the range of common cell lines and tissues available to us. These antibodies are affinity purified using their peptide immunogen and are known to give low background staining in a western blot (see Application Notes below). However no additional claims are made for their ability to recognise native protein in any application. LRP6 is essential for the Wnt/beta catenin signaling pathway, probably by acting as a coreceptor together with Frizzled for Wnt. It is a specific, high-affinity receptor for DKK1 and DKK2, but not DKK3. The interaction with DKK1 blocks LRP6-mediated Wnt/beta catenin signaling via LRP6 removal via Kremen proteins-mediated endocytosis.

Long Name

LDL Receptor-related Protein 6

Alternate Names

ADCAD2, FLJ90062, FLJ90421, LRP6

Gene Symbol

LRP6

Additional LRP-6 Products

Product Documents for Human LRP-6 ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Human LRP-6 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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