Lumican Antibody (4Z2C1)

Novus Biologicals | Catalog # NBP3-15408

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 4Z2C1 expressed in HEK293
View all Lumican Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 212-338 of human Lumican (NP_002336.1). LDNNKISNIPDEYFKRFNALQYLRLSHNELADSGIPGNSFNVSSLVELDLSYNKLKNIPTVNENLENYYLEVNQLEKFDIKSFCKILGPLSYSKIKHLRLDGNRISETSLPPDMYECLRVANEVTLN

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit Lumican Antibody (4Z2C1) (NBP3-15408) is a recombinant monoclonal antibody validated for use in IHC, WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Lumican Antibody (4Z2C1)

Western Blot: Lumican Antibody (4Z2C1) [NBP3-15408]

Western Blot: Lumican Antibody (4Z2C1) [NBP3-15408]

Western Blot: Lumican Antibody (4Z2C1) [NBP3-15408] - Western blot analysis of extracts of various cell lines, using Lumican (LUM) Rabbit mAb (NBP3-15408) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
Immunohistochemistry-Paraffin: Lumican Antibody (4Z2C1) [NBP3-15408]

Immunohistochemistry-Paraffin: Lumican Antibody (4Z2C1) [NBP3-15408]

Immunohistochemistry-Paraffin: Lumican Antibody (4Z2C1) [NBP3-15408] - Immunohistochemistry of paraffin-embedded mouse kidney using Lumican (LUM) Rabbit mAb (NBP3-15408) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: Lumican Antibody (4Z2C1) [NBP3-15408]

Immunohistochemistry-Paraffin: Lumican Antibody (4Z2C1) [NBP3-15408]

Immunohistochemistry-Paraffin: Lumican Antibody (4Z2C1) [NBP3-15408] - Immunohistochemistry of paraffin-embedded human colon using Lumican (LUM) Rabbit mAb (NBP3-15408) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Applications for Lumican Antibody (4Z2C1)

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Immunohistochemistry

1:200 - 1:2000

Immunohistochemistry-Paraffin

1:200 - 1:2000

Western Blot

1:1000 - 1:6000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Lumican

Lumican encodes a member of the small leucine-rich proteoglycan (SLRP) family that includes decorin, biglycan, fibromodulin, keratocan, epiphycan, and osteoglycin. In these bifunctional molecules, the protein moiety binds collagen fibrils and the highly charged hydrophilic glycosaminoglycans regulate interfibrillar spacings. Lumican is the major keratan sulfate proteoglycan of the cornea but is also distributed in interstitial collagenous matrices throughout the body. Lumican may regulate collagen fibril organization and circumferential growth, corneal transparency, and epithelial cell migration and tissue repair. [provided by RefSeq]

Alternate Names

LDC, LUM, SLRR2D

Gene Symbol

LUM

Additional Lumican Products

Product Documents for Lumican Antibody (4Z2C1)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Lumican Antibody (4Z2C1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Proteoglycans

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Protocols

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FAQs

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