Lumican Antibody (JE11-45)

Novus Biologicals | Catalog # NBP2-76847

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JE11-45 expressed in HEK293
View all Lumican Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein within Human Lumican aa 172-338 / 338. (SwissProt: P51884 Human)

Localization

Extracellular matrix. Secreted.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Lumican Antibody (JE11-45)

Western Blot: Lumican Antibody (JE11-45) [NBP2-76847]

Western Blot: Lumican Antibody (JE11-45) [NBP2-76847]

Western Blot: Lumican Antibody (JE11-45) [NBP2-76847] - Western blot analysis of Lumican on different lysates using anti-Lumican antibody at 1/1,000 dilution. Positive control: Lane 1: Jurkat Lane 2: 293T Lane 3: Human skin
Immunocytochemistry/ Immunofluorescence: Lumican Antibody (JE11-45) [NBP2-76847]

Immunocytochemistry/ Immunofluorescence: Lumican Antibody (JE11-45) [NBP2-76847]

Immunocytochemistry/Immunofluorescence: Lumican Antibody (JE11-45) [NBP2-76847] - ICC staining Lumican in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry: Lumican Antibody (JE11-45) [NBP2-76847]

Immunohistochemistry: Lumican Antibody (JE11-45) [NBP2-76847]

Immunohistochemistry: Lumican Antibody (JE11-45) [NBP2-76847] - Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-Lumican antibody. Counter stained with hematoxylin.
Flow Cytometry: Lumican Antibody (JE11-45) [NBP2-76847]

Flow Cytometry: Lumican Antibody (JE11-45) [NBP2-76847]

Flow Cytometry: Lumican Antibody (JE11-45) [NBP2-76847] - Flow cytometric analysis of 293T cells with Lumican antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Immunocytochemistry/ Immunofluorescence: Lumican Antibody (JE11-45) [NBP2-76847]

Immunocytochemistry/ Immunofluorescence: Lumican Antibody (JE11-45) [NBP2-76847]

Immunocytochemistry/Immunofluorescence: Lumican Antibody (JE11-45) [NBP2-76847] - ICC staining Lumican in MG-63 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunocytochemistry/ Immunofluorescence: Lumican Antibody (JE11-45) [NBP2-76847]

Immunocytochemistry/ Immunofluorescence: Lumican Antibody (JE11-45) [NBP2-76847]

Immunocytochemistry/Immunofluorescence: Lumican Antibody (JE11-45) [NBP2-76847] - ICC staining Lumican in PANC-1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry: Lumican Antibody (JE11-45) [NBP2-76847]

Immunohistochemistry: Lumican Antibody (JE11-45) [NBP2-76847]

Immunohistochemistry: Lumican Antibody (JE11-45) [NBP2-76847] - Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-Lumican antibody. Counter stained with hematoxylin.

Applications for Lumican Antibody (JE11-45)

Application
Recommended Usage

Flow Cytometry

1:50-1:100

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:500-1:2000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Lumican

Lumican encodes a member of the small leucine-rich proteoglycan (SLRP) family that includes decorin, biglycan, fibromodulin, keratocan, epiphycan, and osteoglycin. In these bifunctional molecules, the protein moiety binds collagen fibrils and the highly charged hydrophilic glycosaminoglycans regulate interfibrillar spacings. Lumican is the major keratan sulfate proteoglycan of the cornea but is also distributed in interstitial collagenous matrices throughout the body. Lumican may regulate collagen fibril organization and circumferential growth, corneal transparency, and epithelial cell migration and tissue repair. [provided by RefSeq]

Alternate Names

LDC, LUM, SLRR2D

Gene Symbol

LUM

Additional Lumican Products

Product Documents for Lumican Antibody (JE11-45)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Lumican Antibody (JE11-45)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Proteoglycans

Citations for Lumican Antibody (JE11-45)

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Protocols

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FAQs

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