Ly-6A/E Antibody (24GB370)

Name: Ly-6A/E Antibody (24GB370)
Brand: Novus Biologicals
SKU: NBP3-50526
Price: 379 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-50526

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Mouse

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 24GB370

View all Ly-6A/E Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from mouse LLy-6A/E (Uniprot: P05533)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Ly-6A/E Antibody (24GB370)

Immunocytochemistry/ Immunofluorescence: Ly-6A/E Antibody (24GB370)[NBP3-50526] -

Immunocytochemical staining of C2C12 cells with Ly-6A/E antibody (1:1,000). Nuclei were stained blue with DAPI; Ly-6A/E was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 um.

Western Blot: Ly-6A/E Antibody (24GB370)[NBP3-50526] -

Western blotting analysis using Ly-6A/E antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.

Applications for Ly-6A/E Antibody (24GB370)

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Ly-6A/E

Ly-6A/E, also known as Sca-1, is a member of the Ly-6 multigene family of type V glycophosphatidylinositol-anchored cell surface proteins. (1-3) It is expressed on multipotent hematopoietic stem cells in bone marrow of mice with both the Ly-6.1 and Ly-6.2 allotypes. (4-6) In the periphery, Ly-6A/E exhibits a pattern of expression which is based on differences between the two allotypes. Ly-6.1 strains (e.g., A, BALB/c, CBA, C3H/He, DBA/1, NZB) have few Ly-6A/E+ resting peripheral lymphocytes, whereas Ly-6.2 strains (e.g., AKR, C57BL, C57BR, C57L, DBA/2, PL, SJL, SWR, 129) have relatively high numbers of Ly-6A/E+ lymphocytes. (1, 7-9) The expression of Ly-6A/E is dramatically upregulated in all strains upon cellular activation. (1, 8, 9) Studies with MAb D7 suggest that Ly-6A/E may be involved in B and T lymphocyte responses, (9-12) and it appears that the antigen may be required for T-cell receptor-mediated T-cell activation. (3, 13)

Alternate Names

Ly 6A/E, Ly6A.2, Ly6A/E, Ly6E.1, Lymphocyte antigen 6 complex locus A, Lymphocyte antigen 6 complex locus E, Retinoic acid induced gene E, RIGE, SCA1, SCA-1, SCA2, Stem cell antigen 1, Stem cell antigen 2, T cell activating protein, TAP, Thymic shared antigen 1, TSA1

Gene Symbol

LY6A

Additional Ly-6A/E Products

Product Documents for Ly-6A/E Antibody (24GB370)

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Certificate of Analysis

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Product Specific Notices for Ly-6A/E Antibody (24GB370)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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