Ly-6E Antibody (HL1933) - Azide and BSA Free
Novus Biologicals | Catalog # NBP3-25553
Recombinant Monoclonal Antibody
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Key Product Details
Validated by
Knockout/Knockdown, Orthogonal Validation
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL1933 expressed in HEK293
Format
Azide and BSA Free
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Product Specifications
Immunogen
Recombinant fragment of human Ly-6E
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Ly-6E Antibody (HL1933) - Azide and BSA Free
Immunohistochemistry-Paraffin: Ly-6E Antibody (HL1933) - Azide and BSA Free [NBP3-25553] -
Immunohistochemistry-Paraffin: Ly-6E Antibody (HL1933) - Azide and BSA Free [NBP3-25553] - LY6E antibody [HL1933] detects LY6E protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded human placenta. LY6E stained by LY6E antibody [HL1933] (NBP3-25553) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Western Blot: Ly-6E Antibody (HL1933) - Azide and BSA Free [NBP3-25553] -
Western Blot: Ly-6E Antibody (HL1933) - Azide and BSA Free [NBP3-25553] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with LY6E antibody [HL1933] (NBP3-25553) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
Western Blot: Ly-6E Antibody (HL1933) - Azide and BSA Free [NBP3-25553] -
Western Blot: Ly-6E Antibody (HL1933) - Azide and BSA Free [NBP3-25553] - Non-transfected (-) and transfected (+) A549 whole cell extract (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with LY6E antibody [HL1933] (NBP3-25553) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: Ly-6E Antibody (HL1933) [NBP3-25553] -
Ly-6E antibody [HL1933] detects Ly-6E protein at cell membrane and cytoplasm by immunofluorescent analysis.Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min.
Green: Ly-6E stained by Ly-6E antibody [HL1933] (NBP3-25553) diluted at 1:200.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.
Blue: Fluoroshield with DAPI.
Applications for Ly-6E Antibody (HL1933) - Azide and BSA Free
Application
Recommended Usage
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Ly6E
Long Name
Lymphocyte Antigen 6 Family Member E
Alternate Names
Ly-6E, RIGE, SCA2, TSA1
Gene Symbol
LY6E
Additional Ly6E Products
Product Documents for Ly-6E Antibody (HL1933) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Ly-6E Antibody (HL1933) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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