Ly-6G/Ly-6C Antibody (NIMP-R14) - Azide Free

Novus Biologicals | Catalog # NB600-1387

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunoassay, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Functional

Cited:

Immunohistochemistry-Paraffin, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # NIMP-R14

Format

Azide Free
View all Ly-6G (Gr-1) Primary Antibodies »

Product Specifications

Immunogen

Purified BALB/c mouse neutrophils

Epitope

This antibody recognizes a currently undetermined structure on the Neutrophil membrane

Specificity

This monoclonal antibody is highly specific for murine Ly-6G and Ly-6C.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Ly-6G/Ly-6C Antibody (NIMP-R14) - Azide Free

Immunohistochemistry: Ly-6G/Ly-6C Antibody (NIMP-R14) [NB600-1387]

Immunohistochemistry: Ly-6G/Ly-6C Antibody (NIMP-R14) [NB600-1387]

Immunohistochemistry: Ly-6G/Ly-6C Antibody (NIMP-R14) [NB600-1387] - Histopathology of lung sections collected at 24 h pbi from mice co-infected with mouse adapted influenza A/Puerto Rico/8/34 (H1N1) (PR8) and type 2 pneumococcal strain D39 variants. Serial lung sections were subjected to immunohistochemistry (IHC) for pneumococcus using Rabbit Anti-Streptococcus pneumoniae Polyclonal Antibody (Catalog # NB100-64502) at 4 ug/mL or neutrophils using Rat Anti-Ly-6G/Ly-6C Monoclonal Antibody (Catalog # NB600-1387) at 2 ug/mL. Representative images at 4x magnification with 60x magnification inset are shown. Images courtesy of Dr. Amanda P. Smith, UTHSC, TN //doi.org/10.1101/659557
Immunohistochemistry-Frozen: Ly-6G/Ly-6C Antibody (NIMP-R14) [NB600-1387]

Immunohistochemistry-Frozen: Ly-6G/Ly-6C Antibody (NIMP-R14) [NB600-1387]

Immunohistochemistry-Frozen: Ly-6G/Ly-6C Antibody (NIMP-R14) [NB600-1387] - Staining for Ly-6G/Ly-6C FITC of frozen section of mouse spleen.

Applications for Ly-6G/Ly-6C Antibody (NIMP-R14) - Azide Free

Application
Recommended Usage

Flow Cytometry

1:10-1:1000

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:50

Immunohistochemistry-Paraffin

1:50
Application Notes
The monoclonal antibody NIMP-R14 has been succesfully used to stain neutrophils for fluorescent activated cell sorting and in frozen and paraffin sections. ICC/IF reactivity reported in scientific literature (PMID: 32543747).

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS containing 0.1% BSA

Format

Azide Free

Preservative

No Preservative

Concentration

0.1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Ly-6G (Gr-1)

Ly6G is a GPI-anchored protein, that is also known as the myeloid differentiation antigen Gr1. The antigen is transiently expressed on monocytes in the bone marrow. The level of antigen expression in the bone marrow directly correlates with granulocyte differentiation and maturation. Ly6G is expressed predominantly on neutrophils, also in a subset of eosinophils, differentiating pre-monocytes, and plasmacytoid dendritic cells.

Long Name

A Myeloid Differentiation Antigen

Alternate Names

Gr-1

Gene Symbol

LY6G

Additional Ly-6G (Gr-1) Products

Product Documents for Ly-6G/Ly-6C Antibody (NIMP-R14) - Azide Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Ly-6G/Ly-6C Antibody (NIMP-R14) - Azide Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Ly-6G/Ly-6C Antibody (NIMP-R14) - Azide Free

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Protocols

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FAQs for Ly-6G/Ly-6C Antibody (NIMP-R14) - Azide Free

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  • Q: I want to know whether the LY-6G6C Antibody(NIMP-R14) has alternate name,such as "Gr 1"

    A: Our Ly-6G6C antibody with catalogue number NB600-1387 was raised against purified BALB/c mouse neutrophils, and is highly specific for murine Ly-6G and Ly-6C. The Ly-6G/-6C locus encodes a family of Ly-6 proteins which includes Ly-6G and Ly-6C. Ly-6 antigens have a molecular weight of between 15,000 and 18,000. Ly6G is together with Ly6C a component of the myeloid differentiation antigen Gr-1.

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