LY6G6D Antibody (3D6E10) - BSA Free

Novus Biologicals | Catalog # NBP3-27164

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Monkey

Applications

Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 3D6E10

Format

BSA Free
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Product Specifications

Immunogen

Purified recombinant fragment of human LY6G6D (AA: 20-104) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

13.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for LY6G6D Antibody (3D6E10) - BSA Free

Flow Cytometry: Gr-1/Ly-6G Antibody (3D6E10) [NBP3-27164] -

Flow Cytometry: Gr-1/Ly-6G Antibody (3D6E10) [NBP3-27164] - Analysis of Jurkat cells using Gr-1/Ly-6G mouse mAb (green) and negative control (red).

Western Blot: Gr-1/Ly-6G Antibody (3D6E10) [NBP3-27164] -

Western Blot: Gr-1/Ly-6G Antibody (3D6E10) [NBP3-27164] - Analysis using Gr-1/Ly-6G mouse mAb against Jurkat (1), MCF-7 (2), A549 (3),K562 (4),Hela (5),C6 (6),COS-7 (7),and NIH/3T3 (8) cell lysate.

Western Blot: Gr-1/Ly-6G Antibody (3D6E10) [NBP3-27164] -

Western Blot: Gr-1/Ly-6G Antibody (3D6E10) [NBP3-27164] - Analysis using Gr-1/Ly-6G mAb against HEK293-6e (1) and Gr-1/Ly-6G (AA: 20-104)-hIgGFc transfected HEK293-6e (2) cell lysate.

ELISA: Gr-1/Ly-6G Antibody (3D6E10) [NBP3-27164] -

ELISA: Gr-1/Ly-6G Antibody (3D6E10) [NBP3-27164] - Black line: Control Antigen (100 ng); Purple line: Antigen (10 ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)

Western Blot: Gr-1/Ly-6G Antibody (3D6E10) [NBP3-27164] -

Western Blot: Gr-1/Ly-6G Antibody (3D6E10) [NBP3-27164] - Analysis using Gr-1/Ly-6G mAb against human Gr-1/Ly-6G (AA: 20-104) recombinant protein. (Expected MW is 21.4 kDa)

Immunocytochemistry/Immunofluorescence: Gr-1/Ly-6G Antibody (3D6E10) [NBP3-27164] -

Immunocytochemistry/Immunofluorescence: Gr-1/Ly-6G Antibody (3D6E10) [NBP3-27164] - Analysis of Hela cells using Gr-1/Ly-6G mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Applications for LY6G6D Antibody (3D6E10) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunocytochemistry/ Immunofluorescence

1:200 - 1:1000

Western Blot

1:500 - 1:2000

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: LY6G6D

LY6G6D belongs to a cluster of leukocyte antigen-6 (LY6) genes located in the major histocompatibility complex (MHC) class III region on chromosome 6. Members of the LY6 superfamily typically contain 70 to 80 amino acids, including 8 to 10 cysteines. Most LY6 proteins are attached to the cell surface by a glycosylphosphatidylinositol (GPI) anchor that is directly involved in signal transduction (Mallya et al., 2002 [PubMed 12079290]).[supplied by OMIM, Apr 2009]

Alternate Names

C6orf23, Chromosome 6 Open Reading Frame 23, G6D, LY6-D, LY6G6D, Lymphocyte Antigen 6 Complex Locus Protein G6d, Lymphocyte Antigen 6 Complex, Locus G6D, Lymphocyte Antigen-6 G6D, Megakaryocyte-Enhanced Gene Transcript 1 Protein, MEGT1, NG25, Protein Ly6-D

Gene Symbol

LY6G6D

Additional LY6G6D Products

Product Documents for LY6G6D Antibody (3D6E10) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for LY6G6D Antibody (3D6E10) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for LY6G6D Antibody (3D6E10) - BSA Free

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Protocols

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FAQs

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