LYAG/GAA Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-38142

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (91%), Rat (93%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Lysosomal alpha-Glucosidase Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: VGYPFMPPYWGLGFHLCRWGYSSTAITRQVVENMTRAHFPLDVQWNDLDYMDSRRDFTFNKDGFRDFPAMVQELHQGGRRYMMIVDPAISSSGPAGSYRPYDEGLRRGVFITNETGQPLIGKVWPGSTAFPDFTNPTA

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for LYAG/GAA Antibody - BSA Free

Immunohistochemistry-Paraffin: LYAG/GAA Antibody [NBP2-38142]

Immunohistochemistry-Paraffin: LYAG/GAA Antibody [NBP2-38142]

Immunohistochemistry-Paraffin: LYAG/GAA Antibody [NBP2-38142] - Analysis in human placenta and tonsil tissues. Corresponding GAA RNA-seq data are presented for the same tissues.
Western Blot: LYAG/GAA Antibody [NBP2-38142]

Western Blot: LYAG/GAA Antibody [NBP2-38142]

Western Blot: LYAG/GAA Antibody [NBP2-38142] - Analysis in control (vector only transfected HEK293T lysate) and GAA over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: LYAG/GAA Antibody [NBP2-38142]

Immunohistochemistry-Paraffin: LYAG/GAA Antibody [NBP2-38142]

Immunohistochemistry-Paraffin: LYAG/GAA Antibody [NBP2-38142] - Staining of human prostate shows moderate positivity in lysosomes in glandular cells.
Immunohistochemistry-Paraffin: LYAG/GAA Antibody [NBP2-38142]

Immunohistochemistry-Paraffin: LYAG/GAA Antibody [NBP2-38142]

Immunohistochemistry-Paraffin: LYAG/GAA Antibody [NBP2-38142] - Staining of human pancreas shows weak to moderate positivity in lysosomes in exocrine glandular cells.
Immunohistochemistry-Paraffin: LYAG/GAA Antibody [NBP2-38142]

Immunohistochemistry-Paraffin: LYAG/GAA Antibody [NBP2-38142]

Immunohistochemistry-Paraffin: LYAG/GAA Antibody [NBP2-38142] - Staining of human placenta shows weak to moderate positivity in lysosomes in trophoblastic cells.
Immunohistochemistry-Paraffin: LYAG/GAA Antibody [NBP2-38142]

Immunohistochemistry-Paraffin: LYAG/GAA Antibody [NBP2-38142]

Immunohistochemistry-Paraffin: LYAG/GAA Antibody [NBP2-38142] - Staining of human tonsil shows no positivity in non-germinal center cells.

Applications for LYAG/GAA Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Lysosomal alpha-Glucosidase

GAA encodes acid alpha-glucosidase, which is essential for the degradation of glycogen to glucose in lysosomes. Different forms of acid alpha-glucosidase are obtained by proteolytic processing. Defects in this gene are the cause of glycogen storage disease II, also known as Pompe's disease, which is an autosomal recessive disorder with a broad clinical spectrum. Three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

Long Name

Glucosidase, Alpha; Acid

Alternate Names

Acid alpha-Glucosidase, Acid Maltase, GAA, LYAG, Lysosomal alphaGlucosidase

Gene Symbol

GAA

UniProt

P10253

Additional Lysosomal alpha-Glucosidase Products

Product Documents for LYAG/GAA Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for LYAG/GAA Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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Associated Pathways

VEGF - VEGF R2 Signaling Pathways VEGF - VEGF R2 Signaling Pathway Thumbnail