Lyn Antibody (LYN-01) - BSA Free

Novus Biologicals | Catalog # NB500-519

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # LYN-01

Format

BSA Free
View all Lyn Primary Antibodies »

Product Specifications

Immunogen

Bacterially expressed recombinant fragment of human Lyn, aa 8 - 238 (NM_001111097.2).

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

The LYN-01 reacts with Lyn (p56/p53), a non-receptor Src-family tyrosine kinase expressed in hematopoietic tissues.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Lyn Antibody (LYN-01) - BSA Free

Western Blot: Lyn Antibody (LYN-01)BSA Free [NB500-519]

Western Blot: Lyn Antibody (LYN-01)BSA Free [NB500-519]

Western Blot: Lyn Antibody (LYN-01) [NB500-519] - Fig. 1. Western Blotting analysis (non-reducing conditions) of Lyn using anti-Lyn (LYN-01). Lane 1: negative control Lane 2: RBL rat basophilic leukemia cell line Lane 3: JURKAT human peripheral blood T cell leukemia cell line Lane 4: A-431 human epidermoid carcinoma cell line Lane 5: U-937 human histiocytic lymphoma cell line.
Lyn Antibody (LYN-01) - BSA Free Western Blot: Lyn Antibody (LYN-01) - BSA Free [NB500-519]

Western Blot: Lyn Antibody (LYN-01) - BSA Free [NB500-519]

Analysis of human LYN using mouse monoclonal antibody LYN-01 on lysates of Raji cell line and Jurkat cell line (LYN non-expressing cell line; negative control) under non-reducing and reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-LYN monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. LYN was detected around 55 kDa.

Applications for Lyn Antibody (LYN-01) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:2000

Immunoprecipitation

1:50

Western Blot

1-2 ug/ml
Application Notes
For WB: Use non-reducing conditions. Purity is > 95% (by SDS-PAGE).

Reviewed Applications

Read 1 review rated 4 using NB500-519 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

Phosphate buffered saline (PBS), pH 7.4

Format

BSA Free

Preservative

15mM Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Lyn

Lyn is a member of the src family of non-receptor protein tyrosine kinases that is predominantly expressed in haematopoietic tissues. Like all members of the src family, lyn is thought to participate in signal transduction from cell surface receptors that lack intrinsic tyrosine kinase activity. It is associated with a number of cell surface receptors including the B cell antigen receptor and immunoglobulin E receptor (FceRI).

Long Name

V-Yes-1 Yamaguchi Sarcoma Viral Related Oncogene Homolog

Alternate Names

Hck-2, JTK8, v-yes-1

Entrez Gene IDs

4067 (Human)

Gene Symbol

LYN

UniProt

P07948

Additional Lyn Products

Product Documents for Lyn Antibody (LYN-01) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Lyn Antibody (LYN-01) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

B Cell Activation
Intracellular Kinases

Citations for Lyn Antibody (LYN-01) - BSA Free

Customer Reviews for Lyn Antibody (LYN-01) - BSA Free (1)

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  • Name: Hongxia Hu
    Application: Western Blot
    Sample Tested: Hela whole cell lysate
    Species: Human
    Verified Customer | Posted 09/12/2012
    Lyn Antibody (LYN-01) - BSA Free NB500-519

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