Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody (6E10G4) - BSA Free

Novus Biologicals | Catalog # NBP2-52499

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 6E10G4

Format

BSA Free
View all Lysine (K)-specific Demethylase 4A/KDM4A Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A (AA: 932-1057) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

120.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images

Western Blot: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody (6E10G4)BSA Free [NBP2-52499]

Western Blot: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody (6E10G4)BSA Free [NBP2-52499]

Western Blot: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody (6E10G4) [NBP2-52499] - Western blot analysis using KDM4A mAb against human KDM4A (AA: 932-1057) recombinant protein. (Expected MW is 40.6 kDa)
Immunocytochemistry/ Immunofluorescence: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody (6E10G4) - BSA Free [NBP2-52499]

Immunocytochemistry/ Immunofluorescence: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody (6E10G4) - BSA Free [NBP2-52499]

Immunocytochemistry/Immunofluorescence: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody (6E10G4) [NBP2-52499] - Analysis of HeLa cells using KDM4A mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Western Blot: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody (6E10G4)BSA Free [NBP2-52499]

Western Blot: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody (6E10G4)BSA Free [NBP2-52499]

Western Blot: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody (6E10G4) [NBP2-52499] - Analysis using KDM4A mAb against HEK293 (1) and KDM4A (AA: 932-1057)-hIgGFc transfected HEK293 (2) cell lysate.
Immunocytochemistry/ Immunofluorescence: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody (6E10G4) - BSA Free [NBP2-52499]

Immunocytochemistry/ Immunofluorescence: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody (6E10G4) - BSA Free [NBP2-52499]

Immunocytochemistry/Immunofluorescence: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody (6E10G4) [NBP2-52499] - Analysis of HeLa cells using KDM4A mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
ELISA: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody (6E10G4) - BSA Free [NBP2-52499]

ELISA: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody (6E10G4) - BSA Free [NBP2-52499]

ELISA: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody (6E10G4) [NBP2-52499] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);

Applications

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunocytochemistry/ Immunofluorescence

1:200-1:1000

Western Blot

1:500-1:2000
Application Notes
This antibody is Cytof ready.

Flow Cytometry Panel Builder

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Lysine (K)-specific Demethylase 4A/KDM4A

JMJD2A is a member of the Jumonji domain 2 (JMJD2) family and encodes a protein with a JmjN domain, a JmjC domain, a JD2H domain, two TUDOR domains, and two PHD-type zinc fingers. This nuclear protein functions as a trimethylation-specific demethylase, converting specific trimethylated histone residues to the dimethylated form, and as a transcriptional repressor.

Alternate Names

JHDM3A, JMJD2, JMJD2A, KDM4A, Lysine (K)specific Demethylase 4A

Gene Symbol

KDM4A

Additional Lysine (K)-specific Demethylase 4A/KDM4A Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Epigenetics

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Protocols

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