Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody - BSA Free

Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody [NBP1-84352]

Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody [NBP1-84352] - Immunohistochemical staining of human tonsil shows moderate to strong nuclear positivity in lymphoid cells.

Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody [NBP1-84352]

Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody [NBP1-84352] - Immunohistochemical staining of human rectum shows moderate to strong nuclear positivity in glandular cells.

Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody [NBP1-84352]

Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody [NBP1-84352] - Staining of human skeletal muscle shows weak nuclear positivity in myocytes.

Western Blot: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody - BSA Free [NBP1-84352] -

SKP2 regulates the ubiquitination of JARID1B through TRAF6(A) Immunofluorescence images show a co-localization of endogenous JARID1B and TRAF6 in PC3 cells. Scale bars represent 10 μm. (B) and (C) Co-immunoprecipitation analysis shows that endogenous TRAF6 physically interacts with JARID1B in PC3 cells, as shown by reciprocal co-immunoprecipitation between the two proteins (Also see Supplementary Figure S5). (D)In vivo ubiquitination assay shows that TRAF6 increases K63-linked ubiquitination of JARID1B and SKP2 inhibits TRAF6-mediated JARID1B ubiquitination. Cells were transfected with Flag-JARID1B, HA-Ub-K63-only, Myc-TRAF6 and Myc-SKP2 constructs as indicated. WCL indicates the whole cell lysates. (E) TRAF6 mediates JARID1B ubiquitination through lysine residue 242. HEK293T cells were transfected with Flag-JARID1B WT or Flag-JARID1B-K242R, HA-Ub-K63-only, Myc-TRAF6 and Myc-SKP2 plasmids as indicated. In vivo ubiquitination assay was performed in a standard procedure. WCL indicates the whole cell lysates. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/25596733), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody - BSA Free [NBP1-84352] -

SKP2 regulates the ubiquitination of JARID1B through TRAF6(A) Immunofluorescence images show a co-localization of endogenous JARID1B and TRAF6 in PC3 cells. Scale bars represent 10 μm. (B) and (C) Co-immunoprecipitation analysis shows that endogenous TRAF6 physically interacts with JARID1B in PC3 cells, as shown by reciprocal co-immunoprecipitation between the two proteins (Also see Supplementary Figure S5). (D)In vivo ubiquitination assay shows that TRAF6 increases K63-linked ubiquitination of JARID1B and SKP2 inhibits TRAF6-mediated JARID1B ubiquitination. Cells were transfected with Flag-JARID1B, HA-Ub-K63-only, Myc-TRAF6 and Myc-SKP2 constructs as indicated. WCL indicates the whole cell lysates. (E) TRAF6 mediates JARID1B ubiquitination through lysine residue 242. HEK293T cells were transfected with Flag-JARID1B WT or Flag-JARID1B-K242R, HA-Ub-K63-only, Myc-TRAF6 and Myc-SKP2 plasmids as indicated. In vivo ubiquitination assay was performed in a standard procedure. WCL indicates the whole cell lysates. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/25596733), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody - BSA Free [NBP1-84352] -

SKP2 regulates the ubiquitination of JARID1B through TRAF6(A) Immunofluorescence images show a co-localization of endogenous JARID1B and TRAF6 in PC3 cells. Scale bars represent 10 μm. (B) and (C) Co-immunoprecipitation analysis shows that endogenous TRAF6 physically interacts with JARID1B in PC3 cells, as shown by reciprocal co-immunoprecipitation between the two proteins (Also see Supplementary Figure S5). (D)In vivo ubiquitination assay shows that TRAF6 increases K63-linked ubiquitination of JARID1B and SKP2 inhibits TRAF6-mediated JARID1B ubiquitination. Cells were transfected with Flag-JARID1B, HA-Ub-K63-only, Myc-TRAF6 and Myc-SKP2 constructs as indicated. WCL indicates the whole cell lysates. (E) TRAF6 mediates JARID1B ubiquitination through lysine residue 242. HEK293T cells were transfected with Flag-JARID1B WT or Flag-JARID1B-K242R, HA-Ub-K63-only, Myc-TRAF6 and Myc-SKP2 plasmids as indicated. In vivo ubiquitination assay was performed in a standard procedure. WCL indicates the whole cell lysates. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/25596733), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody - BSA Free [NBP1-84352] -

SKP2 regulates the ubiquitination of JARID1B through TRAF6(A) Immunofluorescence images show a co-localization of endogenous JARID1B and TRAF6 in PC3 cells. Scale bars represent 10 μm. (B) and (C) Co-immunoprecipitation analysis shows that endogenous TRAF6 physically interacts with JARID1B in PC3 cells, as shown by reciprocal co-immunoprecipitation between the two proteins (Also see Supplementary Figure S5). (D)In vivo ubiquitination assay shows that TRAF6 increases K63-linked ubiquitination of JARID1B and SKP2 inhibits TRAF6-mediated JARID1B ubiquitination. Cells were transfected with Flag-JARID1B, HA-Ub-K63-only, Myc-TRAF6 and Myc-SKP2 constructs as indicated. WCL indicates the whole cell lysates. (E) TRAF6 mediates JARID1B ubiquitination through lysine residue 242. HEK293T cells were transfected with Flag-JARID1B WT or Flag-JARID1B-K242R, HA-Ub-K63-only, Myc-TRAF6 and Myc-SKP2 plasmids as indicated. In vivo ubiquitination assay was performed in a standard procedure. WCL indicates the whole cell lysates. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/25596733), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunofluorescence: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody - BSA Free [NBP1-84352] -

SKP2 regulates the ubiquitination of JARID1B through TRAF6(A) Immunofluorescence images show a co-localization of endogenous JARID1B and TRAF6 in PC3 cells. Scale bars represent 10 μm. (B) and (C) Co-immunoprecipitation analysis shows that endogenous TRAF6 physically interacts with JARID1B in PC3 cells, as shown by reciprocal co-immunoprecipitation between the two proteins (Also see Supplementary Figure S5). (D)In vivo ubiquitination assay shows that TRAF6 increases K63-linked ubiquitination of JARID1B and SKP2 inhibits TRAF6-mediated JARID1B ubiquitination. Cells were transfected with Flag-JARID1B, HA-Ub-K63-only, Myc-TRAF6 and Myc-SKP2 constructs as indicated. WCL indicates the whole cell lysates. (E) TRAF6 mediates JARID1B ubiquitination through lysine residue 242. HEK293T cells were transfected with Flag-JARID1B WT or Flag-JARID1B-K242R, HA-Ub-K63-only, Myc-TRAF6 and Myc-SKP2 plasmids as indicated. In vivo ubiquitination assay was performed in a standard procedure. WCL indicates the whole cell lysates. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/25596733), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunofluorescence: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody - BSA Free [NBP1-84352] -

SKP2 inactivation induces an accumulation of ubiquitinated JARID1B in nucleolus of cells in vitro and in vivo for cellular senescence(A) Immunofluorescence (IF) images show a co-localization of endogenous JARID1B and Fibrillarin (FBL) in nucleoli of PC3 cells upon SKP2 knockdown (Also see Supplementary Figure S6A). FBL indicates a nucleolar marker. Right panel: quantification of PC3 cells showing an increase of JARID1B localization in nucleolus. Error bars represent means +/- SD. (B) IF images show a co-localization of endogenous JARID1B and K63-Ub in nucleoli of PC3 cells upon SKP2 knockdown. (C) Western blotting assay shows an increase of beta -galactosidase ( beta -Gal) in PC3 cells upon SKP2 knockdown. (D) IF images show JARID1B in nucleolus as indicated by arrows and beta -Gal in cytoplasm in senescent cells upon SKP2 knockdown. (E) The co-localization of endogenous JARID1B and Fibrillarin (FBL) in nucleoli of prostate tissues in Ptenpc−/−;Trp53pc−/−;Skp2−/− mutant mice (Also see Supplementary Figure S6B). Scale bars represent 10 μm for panel A, B, D and E. (F) The positive staining of beta -galactosidase in prostate tissues of Ptenpc−/−;Trp53pc−/−;Skp2−/− mice but not in that of Ptenpc−/−;Trp53pc−/− mice. Scale bars represent 50 μm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/25596733), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunofluorescence: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody - BSA Free [NBP1-84352] -

SKP2 inactivation induces an accumulation of ubiquitinated JARID1B in nucleolus of cells in vitro and in vivo for cellular senescence(A) Immunofluorescence (IF) images show a co-localization of endogenous JARID1B and Fibrillarin (FBL) in nucleoli of PC3 cells upon SKP2 knockdown (Also see Supplementary Figure S6A). FBL indicates a nucleolar marker. Right panel: quantification of PC3 cells showing an increase of JARID1B localization in nucleolus. Error bars represent means +/- SD. (B) IF images show a co-localization of endogenous JARID1B and K63-Ub in nucleoli of PC3 cells upon SKP2 knockdown. (C) Western blotting assay shows an increase of beta -galactosidase ( beta -Gal) in PC3 cells upon SKP2 knockdown. (D) IF images show JARID1B in nucleolus as indicated by arrows and beta -Gal in cytoplasm in senescent cells upon SKP2 knockdown. (E) The co-localization of endogenous JARID1B and Fibrillarin (FBL) in nucleoli of prostate tissues in Ptenpc−/−;Trp53pc−/−;Skp2−/− mutant mice (Also see Supplementary Figure S6B). Scale bars represent 10 μm for panel A, B, D and E. (F) The positive staining of beta -galactosidase in prostate tissues of Ptenpc−/−;Trp53pc−/−;Skp2−/− mice but not in that of Ptenpc−/−;Trp53pc−/− mice. Scale bars represent 50 μm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/25596733), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunofluorescence: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody - BSA Free [NBP1-84352] -

SKP2 inactivation induces an accumulation of ubiquitinated JARID1B in nucleolus of cells in vitro and in vivo for cellular senescence(A) Immunofluorescence (IF) images show a co-localization of endogenous JARID1B and Fibrillarin (FBL) in nucleoli of PC3 cells upon SKP2 knockdown (Also see Supplementary Figure S6A). FBL indicates a nucleolar marker. Right panel: quantification of PC3 cells showing an increase of JARID1B localization in nucleolus. Error bars represent means +/- SD. (B) IF images show a co-localization of endogenous JARID1B and K63-Ub in nucleoli of PC3 cells upon SKP2 knockdown. (C) Western blotting assay shows an increase of beta -galactosidase ( beta -Gal) in PC3 cells upon SKP2 knockdown. (D) IF images show JARID1B in nucleolus as indicated by arrows and beta -Gal in cytoplasm in senescent cells upon SKP2 knockdown. (E) The co-localization of endogenous JARID1B and Fibrillarin (FBL) in nucleoli of prostate tissues in Ptenpc−/−;Trp53pc−/−;Skp2−/− mutant mice (Also see Supplementary Figure S6B). Scale bars represent 10 μm for panel A, B, D and E. (F) The positive staining of beta -galactosidase in prostate tissues of Ptenpc−/−;Trp53pc−/−;Skp2−/− mice but not in that of Ptenpc−/−;Trp53pc−/− mice. Scale bars represent 50 μm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/25596733), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunofluorescence: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody - BSA Free [NBP1-84352] -

SKP2 inactivation induces an accumulation of ubiquitinated JARID1B in nucleolus of cells in vitro and in vivo for cellular senescence(A) Immunofluorescence (IF) images show a co-localization of endogenous JARID1B and Fibrillarin (FBL) in nucleoli of PC3 cells upon SKP2 knockdown (Also see Supplementary Figure S6A). FBL indicates a nucleolar marker. Right panel: quantification of PC3 cells showing an increase of JARID1B localization in nucleolus. Error bars represent means +/- SD. (B) IF images show a co-localization of endogenous JARID1B and K63-Ub in nucleoli of PC3 cells upon SKP2 knockdown. (C) Western blotting assay shows an increase of beta -galactosidase ( beta -Gal) in PC3 cells upon SKP2 knockdown. (D) IF images show JARID1B in nucleolus as indicated by arrows and beta -Gal in cytoplasm in senescent cells upon SKP2 knockdown. (E) The co-localization of endogenous JARID1B and Fibrillarin (FBL) in nucleoli of prostate tissues in Ptenpc−/−;Trp53pc−/−;Skp2−/− mutant mice (Also see Supplementary Figure S6B). Scale bars represent 10 μm for panel A, B, D and E. (F) The positive staining of beta -galactosidase in prostate tissues of Ptenpc−/−;Trp53pc−/−;Skp2−/− mice but not in that of Ptenpc−/−;Trp53pc−/− mice. Scale bars represent 50 μm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/25596733), licensed under a CC-BY license. Not internally tested by Novus Biologicals.