Lysyl Oxidase Homolog 2/LOXL2 Antibody (JU32-53)

Novus Biologicals | Catalog # NBP2-75559

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JU32-53 expressed in HEK293
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Product Specifications

Immunogen

Synthetic peptide within C-terminal human Lysyl Oxidase Homolog 2/LOXL2. (SwissProt: Q9Y4K0 Human; SwissProt: P58022 Mouse; SwissProt: B5DF27 Rat)

Localization

Secreted, Nucleus.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

87 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Lysyl Oxidase Homolog 2/LOXL2 Antibody (JU32-53)

Western Blot: Lysyl Oxidase Homolog 2/LOXL2 Antibody (JU32-53) [NBP2-75559]

Western Blot: Lysyl Oxidase Homolog 2/LOXL2 Antibody (JU32-53) [NBP2-75559]

Western Blot: Lysyl Oxidase Homolog 2/LOXL2 Antibody (JU32-53) [NBP2-75559] - Analysis of LOXL2 on PC-12 and A549 cell lysates using anti-LOXL2 antibody at 1/500 dilution.
Immunocytochemistry/ Immunofluorescence: Lysyl Oxidase Homolog 2/LOXL2 Antibody (JU32-53) [NBP2-75559]

Immunocytochemistry/ Immunofluorescence: Lysyl Oxidase Homolog 2/LOXL2 Antibody (JU32-53) [NBP2-75559]

Immunocytochemistry/Immunofluorescence: Lysyl Oxidase Homolog 2/LOXL2 Antibody (JU32-53) [NBP2-75559] - Staining LOXL2 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: Lysyl Oxidase Homolog 2/LOXL2 Antibody (JU32-53) [NBP2-75559]

Immunohistochemistry-Paraffin: Lysyl Oxidase Homolog 2/LOXL2 Antibody (JU32-53) [NBP2-75559]

Immunohistochemistry-Paraffin: Lysyl Oxidase Homolog 2/LOXL2 Antibody (JU32-53) [NBP2-75559] - Analysis of paraffin-embedded rat brain tissue using anti-LOXL2 antibody. Counter stained with hematoxylin.
Flow Cytometry: Lysyl Oxidase Homolog 2/LOXL2 Antibody (JU32-53) [NBP2-75559]

Flow Cytometry: Lysyl Oxidase Homolog 2/LOXL2 Antibody (JU32-53) [NBP2-75559]

Flow Cytometry: Lysyl Oxidase Homolog 2/LOXL2 Antibody (JU32-53) [NBP2-75559] - Analysis of A549 cells with LOXL2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Immunocytochemistry/ Immunofluorescence: Lysyl Oxidase Homolog 2/LOXL2 Antibody (JU32-53) [NBP2-75559]

Immunocytochemistry/ Immunofluorescence: Lysyl Oxidase Homolog 2/LOXL2 Antibody (JU32-53) [NBP2-75559]

Immunocytochemistry/Immunofluorescence: Lysyl Oxidase Homolog 2/LOXL2 Antibody (JU32-53) [NBP2-75559] - Staining LOXL2 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunocytochemistry/ Immunofluorescence: Lysyl Oxidase Homolog 2/LOXL2 Antibody (JU32-53) [NBP2-75559]

Immunocytochemistry/ Immunofluorescence: Lysyl Oxidase Homolog 2/LOXL2 Antibody (JU32-53) [NBP2-75559]

Immunocytochemistry/Immunofluorescence: Lysyl Oxidase Homolog 2/LOXL2 Antibody (JU32-53) [NBP2-75559] - Staining LOXL2 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: Lysyl Oxidase Homolog 2/LOXL2 Antibody (JU32-53) [NBP2-75559]

Immunohistochemistry-Paraffin: Lysyl Oxidase Homolog 2/LOXL2 Antibody (JU32-53) [NBP2-75559]

Immunohistochemistry-Paraffin: Lysyl Oxidase Homolog 2/LOXL2 Antibody (JU32-53) [NBP2-75559] - Analysis of paraffin-embedded human placenta tissue using anti-LOXL2 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: Lysyl Oxidase Homolog 2/LOXL2 Antibody (JU32-53) [NBP2-75559]

Immunohistochemistry-Paraffin: Lysyl Oxidase Homolog 2/LOXL2 Antibody (JU32-53) [NBP2-75559]

Immunohistochemistry-Paraffin: Lysyl Oxidase Homolog 2/LOXL2 Antibody (JU32-53) [NBP2-75559] - Analysis of paraffin-embedded mouse testis tissue using anti-LOXL2 antibody. Counter stained with hematoxylin.

Applications for Lysyl Oxidase Homolog 2/LOXL2 Antibody (JU32-53)

Application
Recommended Usage

Flow Cytometry

1:50-1:100

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry-Paraffin

1:50-1:100

Western Blot

1:500-1:2000

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Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Lysyl Oxidase Homolog 2/LOXL2

LOXL2 encodes a member of the lysyl oxidase gene family. The prototypic member of the family is essential to the biogenesis of connective tissue, encoding an extracellular copper-dependent amine oxidase that catalyses the first step in the formation of crosslinks in collagens and elastin. A highly conserved amino acid sequence at the C-terminus end appears to be sufficient for amine oxidase activity, suggesting that each family member may retain this function. The N-terminus is poorly conserved and may impart additional roles in developmental regulation, senescence, tumor suppression, cell growth control, and chemotaxis to each member of the family. [provided by RefSeq]

Alternate Names

LOL2, LOXL2

Gene Symbol

LOXL2

Additional Lysyl Oxidase Homolog 2/LOXL2 Products

Product Documents for Lysyl Oxidase Homolog 2/LOXL2 Antibody (JU32-53)

Certificate of Analysis

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Product Specific Notices for Lysyl Oxidase Homolog 2/LOXL2 Antibody (JU32-53)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Lysyl Oxidase Homolog 2/LOXL2 Antibody (JU32-53)

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Protocols

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