M-CSFR/CD115 Antibody

Novus Biologicals | Catalog # NBP1-52070

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all M-CSF R/CD115 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-KNIHLEKKYVR corresponding to internal region according to NP_005202.2.

Epitope

C-KNIHLEKKYVR

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for M-CSFR/CD115 Antibody

Western Blot: M-CSFR/CD115 Antibody [NBP1-52070]

Western Blot: M-CSFR/CD115 Antibody [NBP1-52070]

Western Blot: M-CSF R/CD115 Antibody [NBP1-52070] - HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human CSF1R with C-terminal MYC tag probed with EB10257 (0.5ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with EB10257 (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.

Applications for M-CSFR/CD115 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:32000

Western Blot

0.1 - 0.5 ug/ml
Application Notes
WB: In transfected HEK293 transiently expressing full-length human CSF1R (myc and DYKDDDDK tagged), a band of approx. 200 kDa band was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-myc tag antibody.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: M-CSF R/CD115

MCSF Receptor is the receptor for colony stimulating factor 1, a cytokine which controls the production, differentiation, and function of macrophages. This receptor mediates most if not all of the biological effects of this cytokine. Ligand binding activates the receptor kinase through a process of oligomerization and transphosphorylation. The encoded protein is a member of the CSF1/PDGF receptor family of tyrosine protein kinases and contains 5 immunoglobulin like C2 type domains. CD115 is expressed by cells of the monocytic lineage and by progenitor cells. Mutations in this gene have been associated with a predisposition to myeloid malignancy.

Long Name

Macrophage Colony Stimulating Factor Receptor

Alternate Names

c-fms, CD115, CSF1R, M-CSFR, MCSFR

Entrez Gene IDs

1436 (Human); 12978 (Mouse); 307403 (Rat)

Gene Symbol

CSF1R

UniProt

NP_005202.2 (Human)

Additional M-CSF R/CD115 Products

Product Documents for M-CSFR/CD115 Antibody

Certificate of Analysis

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Product Specific Notices for M-CSFR/CD115 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for M-CSFR/CD115 Antibody

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Associated Pathways

Dendritic Cell Lineage Development Pathways Dendritic Cell Lineage Development Pathway Thumbnail