M-Ras/R-Ras3 Antibody (17H12) - BSA Free

Novus Biologicals | Catalog # NBP3-26128

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG2A Clone # 17H12 expressed in HEK293

Format

BSA Free
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Product Specifications

Immunogen

Recombinant Human M-Ras/R-Ras3 protein [UniProt O14807]

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for M-Ras/R-Ras3 Antibody (17H12) - BSA Free

M-Ras/R-Ras3 Antibody (26E12)

Immunohistochemistry: M-Ras/R-Ras3 Antibody (26E12) [NBP3-26128] -

Immunohistochemistry: M-Ras/R-Ras3 Antibody (26E12) [NBP3-26128] - Image of M-Ras/R-Ras3 Antibody (26E12) diluted at 1:600 and staining in paraffin-embedded human prostate tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.
M-Ras/R-Ras3 Antibody (26E12)

Immunocytochemistry/Immunofluorescence: M-Ras/R-Ras3 Antibody (26E12) [NBP3-26128] -

Immunocytochemistry/Immunofluorescence: M-Ras/R-Ras3 Antibody (26E12) [NBP3-26128] - Staining of Hela cell with M-Ras/R-Ras3 Antibody (26E12) at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated Goat Anti-Mouse IgG (H+L).

Applications for M-Ras/R-Ras3 Antibody (17H12) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry

1:50-1:200

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50% Glycerol, 0.01M PBS, PH 7.4

Format

BSA Free

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: M-Ras/R-Ras3

MRAS (muscle RAS oncogene homolog) belongs to the Ras family of small GTPases and could play a role as a signal transducer. MRAS is known to have interactions with MLLT4, RASSF5, RALGDS, BRAF and PIK3CA. This protein is currently being studied for research on the following diseases and disorders: neuronitis, Parkinson's disease, gigantism and Noonan syndrome.

Alternate Names

MRas, R-RAS3

Gene Symbol

MRAS

Additional M-Ras/R-Ras3 Products

Product Documents for M-Ras/R-Ras3 Antibody (17H12) - BSA Free

Certificate of Analysis

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Product Specific Notices for M-Ras/R-Ras3 Antibody (17H12) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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