MafB Antibody (BLR046F)

Novus Biologicals | Catalog # NBP3-14673

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit Clone # BLR046F
View all MafB Primary Antibodies »

Product Specifications

Immunogen

between 1 and 50

Clonality

Monoclonal

Host

Rabbit

Scientific Data Images for MafB Antibody (BLR046F)

Western Blot: MafB Antibody (BLR046F) [NBP3-14673]

Western Blot: MafB Antibody (BLR046F) [NBP3-14673]

Western Blot: MafB Antibody [NBP3-14673] - Antibody: Rabbit anti-MafB recombinant monoclonal antibody used at 1:1000. Secondary: HRPconjugated goat anti-rabbit IgGChemiluminescence with an exposure time of 3 minutes.Lower Panels: Rabbit anti-MafA recombinant monoclonalantibody and rabbit anti-Actinrecombinant monoclonal antibody.
Immunohistochemistry: MafB Antibody (BLR046F) [NBP3-14673]

Immunohistochemistry: MafB Antibody (BLR046F) [NBP3-14673]

Immunohistochemistry: MafB Antibody [NBP3-14673] - Rabbit anti-MafBrecombinant monoclonal antibody. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
Western Blot: MafB Antibody (BLR046F) [NBP3-14673]

Western Blot: MafB Antibody (BLR046F) [NBP3-14673]

Western Blot: MafB Antibody [NBP3-14673] - Whole cell lysate (50 ug) from HeLa, HEK293T, SK-N-MC, RPMI-8226, Jurkat, KG-1, K-562, and MOLT-4 cells prepared using NETN lysis buffer. Rabbit anti-MafB recombinant monoclonal antibody [BLR046F] (NBP3-14673 lot 2) used at 1:1000. HRP-conjugated goat anti-rabbit IgG (NB7156). Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-COPB2.
Immunohistochemistry: MafB Antibody (BLR046F) [NBP3-14673]

Immunohistochemistry: MafB Antibody (BLR046F) [NBP3-14673]

Immunohistochemistry: MafB Antibody [NBP3-14673] - Rabbit anti-MafBrecombinant monoclonal antibody. Secondary: HRP-conjugated goat anti-rabbit IgG Substrate: DAB.
Immunoprecipitation: MafB Antibody (BLR046F) [NBP3-14673]

Immunoprecipitation: MafB Antibody (BLR046F) [NBP3-14673]

Immunoprecipitation: MafB Antibody [NBP3-14673] - Samples: Whole cell lysate (1.0 mgper IP reaction; 20% of IP loaded) from SK-N-MC cellsprepared using NETN lysis buffer. Antibodies: Rabbit antiMafB recombinant monoclonal antibody used for IP at 6 ul/mg lysate. MafB wasalso immunoprecipitated by a previous lot of this antibody. For blotting immunoprecipitated MafB, was used at 1:1000. Chemiluminescence withan exposure time of 30 seconds.

Applications for MafB Antibody (BLR046F)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100 to 1:500

Immunohistochemistry

1:100 to 1:500

Immunohistochemistry-Paraffin

1:100 to 1:500

Immunoprecipitation

6 ul/1 mg lysate

Western Blot

1:1000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.

Formulation, Preparation, and Storage

Purification

>95%

Formulation

Borate Buffered Saline (BBS) pH 8.2 with, BSA Free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 2 - 8 C / 1 year from date of receipt

Background: MafB

MAFB is encoded by this gene is a basic leucine zipper (bZIP) transcription factor that plays an important role in the regulation of lineage-specific hematopoiesis. The encoded nuclear protein represses ETS1-mediated transcription of erythroid-specific genes in myeloid cells. This gene contains no introns.

Long Name

v-maf Musculoaponeurotic Fibrosarcoma Oncogene Homolog B

Alternate Names

KRML

Gene Symbol

MAFB

Additional MafB Products

Product Documents for MafB Antibody (BLR046F)

Certificate of Analysis

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Product Specific Notices for MafB Antibody (BLR046F)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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