MAGEA4 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-84365

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all MAGEA4 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: WRQPNEGSSSQEEEGPSTSPDAESLFREALSNKVDELAHFLLRKYRAKELVTKAEMLERVIKNYKRCFPVIFGKASESLKMIFGIDVKEVDPASNTYTLVTCLGLSYDGLLGNNQIFPKTGLLIIVLGT

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MAGEA4 Antibody - BSA Free

Immunohistochemistry-Paraffin: MAGEA4 Antibody [NBP1-84365]

Immunohistochemistry-Paraffin: MAGEA4 Antibody [NBP1-84365]

Immunohistochemistry-Paraffin: MAGEA4 Antibody [NBP1-84365] - Analysis in human testis and endometrium tissues using NBP1-84365 antibody. Corresponding MAGEA4 RNA-seq data are presented for the same tissues.
Immunocytochemistry/ Immunofluorescence: MAGEA4 Antibody [NBP1-84365]

Immunocytochemistry/ Immunofluorescence: MAGEA4 Antibody [NBP1-84365]

Immunocytochemistry/Immunofluorescence: MAGEA4 Antibody [NBP1-84365] - Staining of human cell line U-2 OS shows localization to nuclear speckles & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: MAGEA4 Antibody [NBP1-84365]

Immunohistochemistry-Paraffin: MAGEA4 Antibody [NBP1-84365]

Immunohistochemistry-Paraffin: MAGEA4 Antibody [NBP1-84365] - Staining of human testis shows high expression.
MAGEA4 Antibody - BSA Free Immunohistochemistry-Paraffin: MAGEA4 Antibody - BSA Free [NBP1-84365]

Immunohistochemistry-Paraffin: MAGEA4 Antibody - BSA Free [NBP1-84365]

Staining of human duodenum shows no positivity in glandular cells as expected.
MAGEA4 Antibody - BSA Free Immunohistochemistry-Paraffin: MAGEA4 Antibody - BSA Free [NBP1-84365]

Immunohistochemistry-Paraffin: MAGEA4 Antibody - BSA Free [NBP1-84365]

Staining of human tonsil shows no positivity in lymphoid cells as expected.
MAGEA4 Antibody - BSA Free Immunohistochemistry-Paraffin: MAGEA4 Antibody - BSA Free [NBP1-84365]

Immunohistochemistry-Paraffin: MAGEA4 Antibody - BSA Free [NBP1-84365]

Staining of human endometrium shows no positivity in glandular cells as expected.

Applications for MAGEA4 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MAGEA4

MAGEA4 is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita. At least four variants encoding the same protein have been found for this gene.

Long Name

Melanoma-Associated Antigen 4

Alternate Names

CT1.4, MAGE-41, MAGE-X2, MAGE4A, MAGE4B

Entrez Gene IDs

4103 (Human)

Gene Symbol

MAGEA4

UniProt

P43358 (Human)

Additional MAGEA4 Products

Product Documents for MAGEA4 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for MAGEA4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Stem Cell Markers

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Protocols

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FAQs

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