MAGEB3 Antibody (OTI5H8) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-72579

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI5H8

Format

Azide and BSA Free
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Product Specifications

Immunogen

Full length human recombinant protein of human MAGEB3(NP_002356) produced in E.coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

39 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MAGEB3 Antibody (OTI5H8) - Azide and BSA Free

Western Blot: MAGEB3 Antibody (OTI5H8)Azide and BSA Free [NBP2-72579]

Western Blot: MAGEB3 Antibody (OTI5H8)Azide and BSA Free [NBP2-72579]

Western Blot: MAGEB3 Antibody (OTI5H8) - Azide and BSA Free [NBP2-72579] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAGEB3.

Applications for MAGEB3 Antibody (OTI5H8) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MAGEB3

The MAGEB3 gene is a MAGE-B subfamily member of the MAGE gene family. MAGE family member proteins direct the expression of tumor antigens recognized on a human melanoma by autologous cytolytic T lymphocytes. There are two known clusters of MAGE genes on chromos

Alternate Names

CT3.5, MAGE-B3 antigen, melanoma antigen family B, 3, melanoma-associated antigen B3, member 5

Gene Symbol

MAGEB3

Additional MAGEB3 Products

Product Documents for MAGEB3 Antibody (OTI5H8) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for MAGEB3 Antibody (OTI5H8) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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