MAGI3 Antibody

Western Blot: MAGI3 Antibody [NBP2-17210]

MAGI3-Antibody-Western-Blot-NBP2-17210-img0003.jpg

Western Blot: MAGI3 Antibody [NBP2-17210] -

Western Blot: MAGI3 Antibody [NBP2-17210] - Expression of truncated MAGI3 leads to increased YAP activation & YAP-dependent transformation.(A-B) Immunofluorescence of serum-stimulated HEK293T cells showing YAP (red) localization. GFP positivity indicates full-length MAGI3-transfected cells. DAPI (blue) was used to visualize nuclei. (A) Field containing predominantly untransfected cells is shown. (B) Field containing MAGI3-transfected cells is shown. (C-D) Immunofluorescence of serum-deprived HEK293T cells showing YAP (red) localization. GFP indicates expression & localization of MAGI3 delta C-GFP. DAPI (blue) was used to visualize nuclei. (C) Field containing predominantly untransfected cells is shown. (D) Field containing MAGI3 delta C-GFP-transfected cells is shown. (E) Immunoblots of V5-tagged MAGI3pPA, MAGI3 delta C-GFP, Ser127-phosphorylated YAP, total YAP & beta -actin levels in HEK293T cells transfected as indicated. The relative levels of Ser127-phosphorylated YAP in the indicated lysates are normalized to total YAP levels. (F) Anchorage-independent growth assays for MCF10A-SV40 cells expressing MAGI3 delta C-GFP with or without shYAP1. Images show representative soft agar fields for the indicated cell lines after three weeks. Scale bar represents 100 µm. (G) Quantification of transformed colonies in the indicated cell lines (n = 3 biological replicates per cell line) after three weeks (upper panel). Immunoblots of YAP & beta -actin levels in the cell lines (lower panels). Data are presented as mean ± SEM. ***p<0.001 (two-tailed Student’s t-tests).DOI:http://dx.doi.org/10.7554/eLife.14730.010 Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27205883), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: MAGI3 Antibody [NBP2-17210] -

Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with MAGI3 antibody [N1N2], N-term (NBP2-17210) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.