MANEA Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-83710

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all MANEA Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: GVLALSWYPPDVNDENGEPTDNLVPTILDKAHKYNLKVTFHIEPYSNRDDQNMYKNVKYIIDKYGNHPAFYRYKTKTGNALPMFYVYDSYITKPEKWANLLTTSGSRSIRNSPYDGLFIALLVEEKHKYDILQSGFDGIYT

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (87%), Rat (88%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MANEA Antibody - BSA Free

Immunohistochemistry-Paraffin: MANEA Antibody [NBP1-83710]

Immunohistochemistry-Paraffin: MANEA Antibody [NBP1-83710]

Immunohistochemistry-Paraffin: MANEA Antibody [NBP1-83710] - Staining of human liver.
Immunohistochemistry-Paraffin: MANEA Antibody [NBP1-83710]

Immunohistochemistry-Paraffin: MANEA Antibody [NBP1-83710]

Immunohistochemistry-Paraffin: MANEA Antibody [NBP1-83710] - Staining of human colon, kidney, liver and testis using Anti-MANEA antibody NBP1-83710 (A) shows similar protein distribution across tissues to independent antibody NBP1-82279 (B).
Immunohistochemistry-Paraffin: MANEA Antibody [NBP1-83710]

Immunohistochemistry-Paraffin: MANEA Antibody [NBP1-83710]

Immunohistochemistry-Paraffin: MANEA Antibody [NBP1-83710] - Staining of human testis.
Immunohistochemistry-Paraffin: MANEA Antibody [NBP1-83710]

Immunohistochemistry-Paraffin: MANEA Antibody [NBP1-83710]

Immunohistochemistry-Paraffin: MANEA Antibody [NBP1-83710] - Staining of human kidney.
Immunohistochemistry-Paraffin: MANEA Antibody [NBP1-83710]

Immunohistochemistry-Paraffin: MANEA Antibody [NBP1-83710]

Immunohistochemistry-Paraffin: MANEA Antibody [NBP1-83710] - Staining of human colon.

Applications for MANEA Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MANEA

N-glycosylation of proteins is initiated in the endoplasmic reticulum (ER) by the transfer of the preassembled oligosaccharide glucose-3-mannose-9-N-acetylglucosamine-2 from dolichyl pyrophosphate to acceptor sites on the target protein by an oligosaccharyltransferase complex. This core oligosaccharide is sequentially processed by several ER glycosidases and by an endomannosidase (E.C. 3.2.1.130), such as MANEA, in the Golgi. MANEA catalyzes the release of mono-, di-, and triglucosylmannose oligosaccharides by cleaving the alpha-1,2-mannosidic bond that links them to high-mannose glycans (Hamilton et al., 2005 [PubMed 15677381]).[supplied by OMIM]

Alternate Names

alpha 1,2-endomannosidase, DKFZp686D20120, EC 3.2.1, EC 3.2.1.130, ENDO, Endo-alpha mannosidase, endomannosidase, FLJ12838, glycoprotein endo-alpha-1,2-mannosidase, hEndo, mandaselin, mannosidase, endo-alpha

Gene Symbol

MANEA

Additional MANEA Products

Product Documents for MANEA Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for MANEA Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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