MANF Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-90099

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (97%), Rat (97%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all MANF Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: CEVCISYLGRFYQDLKDRDVTFSPATIENELIKFCREAR

Reactivity Notes

Reactivity reported in scientific literature (PMID: 23435261)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit MANF Antibody - BSA Free (NBP1-90099) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-MANF Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for MANF Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: MANF Antibody [NBP1-90099]

Immunocytochemistry/ Immunofluorescence: MANF Antibody [NBP1-90099]

Immunocytochemistry/Immunofluorescence: ARMET Antibody [NBP1-90099] - Staining of human cell line U-2 OS shows localization to endoplasmic reticulum. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: MANF Antibody [NBP1-90099]

Immunohistochemistry-Paraffin: MANF Antibody [NBP1-90099]

Immunohistochemistry-Paraffin: ARMET Antibody [NBP1-90099] - Staining of human testis shows moderate to strong cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: MANF Antibody [NBP1-90099]

Immunohistochemistry-Paraffin: MANF Antibody [NBP1-90099]

Immunohistochemistry-Paraffin: ARMET Antibody [NBP1-90099] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neurons.
Immunohistochemistry-Paraffin: MANF Antibody [NBP1-90099]

Immunohistochemistry-Paraffin: MANF Antibody [NBP1-90099]

Immunohistochemistry-Paraffin: ARMET Antibody [NBP1-90099] - Staining of human rectum shows moderate cytoplasmic positivity in peripheral ganglia, as well as glandular and lymphoid cells.
Immunohistochemistry-Paraffin: MANF Antibody [NBP1-90099]

Immunohistochemistry-Paraffin: MANF Antibody [NBP1-90099]

Immunohistochemistry-Paraffin: ARMET Antibody [NBP1-90099] - Staining of human skeletal muscle shows no positivity in striated muscle fibers as expected.
MANF Antibody - BSA Free Western Blot: MANF Antibody - BSA Free [NBP1-90099]

Western Blot: MANF Antibody - BSA Free [NBP1-90099]

Analysis in human cell line HepG2.

Applications for MANF Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MANF

MANF is localized in the endoplasmic reticulum (ER) and golgi, and is also secreted. Reducing expression of this gene increases susceptibility to ER stress-induced death and promotes cell proliferation. The protein was initially thought to be longer at the N-terminus and to contain an arginine-rich region but transcribed evidence indicates a smaller open reading frame that does not encode the arginine tract. The presence of a specific mutation changing the previously numbered codon 50 from ATG to AGG, or deletion of that codon, has been reported in a variety of solid tumors. With the protein size correction, this codon is now identified as the initiation codon. [provided by RefSeq]

Long Name

Mesencephalic Astrocyte-derived Neurotrphic Factor

Alternate Names

ARMET, ARP

Gene Symbol

MANF

Additional MANF Products

Product Documents for MANF Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for MANF Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for MANF Antibody - BSA Free

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Protocols

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