MAP3K12 Antibody

Novus Biologicals | Catalog # NBP2-17218

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Simple Western

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all MAP3K12 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of mouse MAP3K12. The exact sequence is proprietary.

Localization

Cytoplasm; Membrane; Peripheral membrane protein

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

96 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MAP3K12 Antibody

Immunohistochemistry-Frozen: MAP3K12 Antibody [NBP2-17218]

Immunohistochemistry-Frozen: MAP3K12 Antibody [NBP2-17218]

Immunohistochemistry-Frozen: MAP3K12 Antibody [NBP2-17218] - Frozen sectioned E13.5 Rat brain. Green: DLK/MAP3K12 protein stained by DLK/MAP3K12 antibody diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI.
Western Blot: MAP3K12 Antibody [NBP2-17218]

Western Blot: MAP3K12 Antibody [NBP2-17218]

Western Blot: MAP3K12 Antibody [NBP2-17218] - Sample (50 ug of whole cell lysate) A: mouse brain 7.5% SDS PAGE diluted at 1:1000.
Immunohistochemistry-Paraffin: MAP3K12 Antibody [NBP2-17218]

Immunohistochemistry-Paraffin: MAP3K12 Antibody [NBP2-17218]

Immunohistochemistry-Paraffin: MAP3K12 Antibody [NBP2-17218] - Analysis of paraffin-embedded Breast ca, using antibody at 1:250 dilution.
MAP3K12 Antibody

Western Blot: MAP3K12 Antibody [NBP2-17218] -

Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DLK/MAP3K12 antibody (NBP2-17218) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
MAP3K12 Antibody

Western Blot: MAP3K12 Antibody [NBP2-17218] -

DLK/MAP3K12 antibody detects DLK/MAP3K12 protein by western blot analysis. Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with DLK/MAP3K12 antibody (NBP2-17218) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
MAP3K12 Antibody

Western Blot: MAP3K12 Antibody [NBP2-17218] -

Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with DLK/MAP3K12 antibody (NBP2-17218) diluted at 1:1000.
MAP3K12 Antibody

Western Blot: MAP3K12 Antibody [NBP2-17218] -

DLK/MAP3K12 antibody detects MAP3K12 protein by Western blot analysis.
A. 20 ug mouse embryonic stem cell whole cell lysate/extract
7.5 % SDS-PAGE
DLK/MAP3K12 antibody (NBP2-17218) dilution: 1:1000

Applications for MAP3K12 Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Frozen

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Application Notes

See Simple Western Antibody Database for Simple Western validation: Tested in Mouse Retinal Ganglion Cells, separated by Size

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: MAP3K12

May be an activator of the JNK/SAPK pathway. Phosphorylates beta-casein, histone 1 and myelin basic protein in vitro.

Alternate Names

DLKEC 2.7.11.25, Dual leucine zipper bearing kinase, EC 2.7.11, Leucine-zipper protein kinase, MAPK-upstream kinase, MEKK12, mitogen-activated protein kinase kinase kinase 12, Mixed lineage kinase, MUKdual leucine zipper kinase DLK, protein kinase MUK, ZPKleucine zipper protein kinase, ZPKP1

Gene Symbol

MAP3K12

UniProt

Q60700 (Mouse)

Additional MAP3K12 Products

Product Documents for MAP3K12 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for MAP3K12 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Citations for MAP3K12 Antibody

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Protocols

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