MAP4K6 Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-32766
![Western Blot: MAP4K6 Antibody [NBP1-32766]](https://resources.rndsystems.com/images/products/MAP4K6-Antibody-Western-Blot-NBP1-32766-img0001.jpg "Western Blot: MAP4K6 Antibody [NBP1-32766]")
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Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human MAP4K6. The exact sequence is proprietary.
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Zebrafish (81%), Xenopus laevis (86%).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
150 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Rabbit MAP4K6 Antibody - BSA Free (NBP1-32766) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for MAP4K6 Antibody - BSA Free
Western Blot: MAP4K6 Antibody [NBP1-32766]
Western Blot: MAP4K6 Antibody [NBP1-32766] - Sample (30 ug of whole cell lysate) A: Hep G2 7.5% SDS PAGE diluted at 1:1000![Immunocytochemistry/ Immunofluorescence: MAP4K6 Antibody [NBP1-32766]](https://resources.rndsystems.com/images/products/MAP4K6-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32766-img0005.jpg "Immunocytochemistry/ Immunofluorescence: MAP4K6 Antibody [NBP1-32766]")
Immunocytochemistry/ Immunofluorescence: MAP4K6 Antibody [NBP1-32766]
Immunocytochemistry/Immunofluorescence: MAP4K6 Antibody [NBP1-32766] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: MAP4K6 protein stained by MAP4K6 antibody [C2C3-2], C-term diluted at 1:500. Blue: Hoechst 33342 staining.![Immunohistochemistry-Paraffin: MAP4K6 Antibody [NBP1-32766]](https://resources.rndsystems.com/images/products/MAP4K6-Antibody-Immunohistochemistry-Paraffin-NBP1-32766-img0004.jpg "Immunohistochemistry-Paraffin: MAP4K6 Antibody [NBP1-32766]")
Immunohistochemistry-Paraffin: MAP4K6 Antibody [NBP1-32766]
Immunohistochemistry-Paraffin: MAP4K6 Antibody [NBP1-32766] - Paraffin-embedded U87 xenograft; antibody dilution 1:500.Applications for MAP4K6 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol
Format
BSA Free
Preservative
0.01% Thimerosal
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: MAP4K6
Alternate Names
B55, EC 2.7.11, GCK family kinase MiNK, hMINK, hMINKbeta, MAP4K6EC 2.7.11.1, MAPK/ERK kinase kinase kinase 6, MEK kinase kinase 6, MGC21111, MINKMEKKK 6, Misshapen/NIK-related kinase, misshapen-like kinase 1, misshapen-like kinase 1 (zebrafish), Mitogen-activated protein kinase kinase kinase kinase 6, serine/threonine protein kinase, YSK2, ZC3
Gene Symbol
MINK1
Additional MAP4K6 Products
Product Documents for MAP4K6 Antibody - BSA Free
Product Specific Notices for MAP4K6 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.Customer Reviews for MAP4K6 Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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