MARCH4 Antibody - BSA Free
Novus Biologicals | Catalog # NBP2-24502
![Western Blot: MARCH4 Antibody [NBP2-24502]](https://resources.rndsystems.com/images/products/MARCH4-Antibody-Western-Blot-NBP2-24502-img0002.jpg "Western Blot: MARCH4 Antibody [NBP2-24502]")
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Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Equine, Primate
Predicted:
Chimpanzee (100%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
A portion of amino acids 300-350 of human RNF174 was used as the immunogen.
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Monkey (88%).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for MARCH4 Antibody - BSA Free
Western Blot: MARCH4 Antibody [NBP2-24502]
Western Blot: MARCH4 Antibody [NBP2-24502] - Analysis of human RNF174 in Jurkat cell lysate in the 1) absence and 2) presence of immunizing peptide using this antibody at 5 ug/ml.![Immunohistochemistry-Paraffin: MARCH4 Antibody [NBP2-24502]](https://resources.rndsystems.com/images/products/MARCH4-Antibody-Immunohistochemistry-Paraffin-NBP2-24502-img0001.jpg "Immunohistochemistry-Paraffin: MARCH4 Antibody [NBP2-24502]")
Immunohistochemistry-Paraffin: MARCH4 Antibody [NBP2-24502]
Immunohistochemistry-Paraffin: MARCH4 Antibody [NBP2-24502] - IHC analysis of human liver using this antibody at 5 ug/ml.Applications for MARCH4 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry-Paraffin
10 ug/ml
Western Blot
0.5-2 ug/ml
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS and 0.2% Gelatin
Format
BSA Free
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: MARCH4
Alternate Names
EC 6.3.2.-, KIAA1399E3 ubiquitin-protein ligase MARCH4, MARCH-IVMGC104908, membrane-associated ring finger (C3HC4) 4, Membrane-associated RING finger protein 4, Membrane-associated RING-CH protein IV, RNF174RING finger protein 174
Gene Symbol
MARCH4
Additional MARCH4 Products
Product Documents for MARCH4 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for MARCH4 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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