MARCKS Antibody (5F9) - BSA Free
Novus Biologicals | Catalog # NBP3-05539
![Western Blot: MARCKS Antibody [NBP3-05539]](https://resources.rndsystems.com/images/products/MARCKS-Antibody-Western-Blot-NBP3-05539-img0002.jpg "Western Blot: MARCKS Antibody [NBP3-05539]")
Key Product Details
Species Reactivity
Human, Monkey
Applications
Immunohistochemistry, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 5F9
Format
BSA Free
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Product Specifications
Immunogen
Full length human MARCKS expressed in and purified from E. coli.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
80 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for MARCKS Antibody (5F9) - BSA Free
Western Blot: MARCKS Antibody [NBP3-05539]
Western Blot: MARCKS Antibody [NBP3-05539] - Western blot analysis of different cell lysates using MARCKS Antibody in green: [1] protein standard, [2] NIH-3T3, [3] HEK293, [4] HeLa, [5] SH-SY5Y, [6] C6 and [7] COS1 cells. The band at about 80kDa corresponds to the MARCKS protein detected only in the human and monkey cells. The antibody does not recognize the rodent form of the MARCKS protein.![Immunocytochemistry/ Immunofluorescence: MARCKS Antibody [NBP3-05539]](https://resources.rndsystems.com/images/products/MARCKS-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05539-img0001.jpg "Immunocytochemistry/ Immunofluorescence: MARCKS Antibody [NBP3-05539]")
Immunocytochemistry/ Immunofluorescence: MARCKS Antibody [NBP3-05539]
Immunocytochemistry/Immunofluorescence: MARCKS Antibody [NBP3-05539] - Immunofluorescent analysis of HeLa cell culture stained with MARCKS Antibody at a dilution of 1:1,000 in red, and costained with chicken pAb to vimentin at a dilution of 1:10,000 in green. The blue is Hoechst staining of nuclear DNA. MARCKS antibody binds to MARCKS protein expressed in the membrane structures and in cytoplasm, while the vimentin antibody stains the intermediate filaments.Applications for MARCKS Antibody (5F9) - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:2000
Immunohistochemistry
1:2000
Western Blot
1:2000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
50% PBS, 50% glycerol
Format
BSA Free
Preservative
0.035% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Store at -20C long term. Avoid freeze-thaw cycles.
Background: MARCKS
Long Name
Myristoylated Alanine-rich Protein Kinase C Substrate
Alternate Names
80K-L, MACS, PRKCSL
Gene Symbol
MARCKS
Additional MARCKS Products
Product Documents for MARCKS Antibody (5F9) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for MARCKS Antibody (5F9) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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