MARCKS Antibody

Western Blot: MARCKS Antibody [NBP3-05540]

Western Blot: MARCKS Antibody [NBP3-05540] - Western blot analysis of different cell lines lysates using MARCKS, at a dilution of 1:1,000 in green: [1] protein standard (red), [2] HEK293, [3] HeLa, [4] SH-SY5Y, and [5] COS1 cells. Strong band at 80kDa corresponds to MARCKS protein. The same blot was simultaneously probed with mouse mAb to GAPDH at a dilution of 1:5,000 in red, which reveals a single band at 37kDa in all preparations.

Immunocytochemistry/ Immunofluorescence: MARCKS Antibody [NBP3-05540]

Immunocytochemistry/Immunofluorescence: MARCKS Antibody [NBP3-05540] - Immunofluorescent analysis of HeLa cells stained with MARCKS Antibody at a dilution of 1:5,000 in red, and costained with mouse mAb to beta-tubulin at a dilution of 1:10,000 in green. The blue is Hoechst staining of nuclear DNA. The MARCKS antibody binds to MARCKS protein expressed in the plasma membrane and cytoplasm, while the beta-tubulin antibody stains cytoplasmic microtubules.