Mast Cell Protease-11 (MCP-11) is encoded by Prss34, one of 13 genes on mouse chromosome 17A3.3 that correspond to functional Trypsin-like serine proteases. Its mRNA is preferentially expressed in spleen and bone marrow. Apparently, the human counterpart is not enzymatically active due to a mutation in the gene that leads to a premature translation termination codon.
Mast Cell Protease-11/Prss34 Antibody (451421) [CoraFluor™ 1]
Novus Biologicals | Catalog # FAB2857CL1
![Mast Cell Protease-11/Prss34 Antibody (451421) [CoraFluor™ 1]](https://resources.rndsystems.com/images/products/fab2857cl1_rat-mast-cell-protease-11-prss34-mab-451421-corafluor-1-259202511203214.png "Product Feature: CoraFluor Probes for TR-FRET")
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Key Product Details
Species Reactivity
Mouse
Applications
Western Blot, Immunoprecipitation
Label
CoraFluor 1
Antibody Source
Monoclonal Rat IgG1 Clone # 451421
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Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Mast Cell Protease‑11/Prss34
Met20-Ser318
Accession # Q80UR4
Met20-Ser318
Accession # Q80UR4
Specificity
Detects mouse Mast Cell Protease‑11/Prss34 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse (rm) MCP-1, rmMCP-6, or rmMCP-7 is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG1
Description
This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
Scientific Data Images for Mast Cell Protease-11/Prss34 Antibody (451421) [CoraFluor™ 1]
Product Feature: CoraFluor Probes for TR-FRET
CoraFluor™ 1, amine reactive (Catalog:7920) and CoraFluor™ 2, amine reactive (Catalog # 7950) are terbium-based probes that have been developed for use as TR-FRET donors. They emit wavelengths compatible with commonly used fluorescent acceptor dyes such as BODIPY® (or BDY) and Janelia Fluor® dyes, FITC (Catalog # 5440), TMR and Cyanine 5 (Catalog # 5436). CoraFluor™ fluorescence is brighter and more stable in biological media than existing TR-FRET donors, leading to enhanced sensitivity and improved data generation. CoraFluor™ 1 exhibits excitation upon exposure to a 337 nm UV laser.Applications for Mast Cell Protease-11/Prss34 Antibody (451421) [CoraFluor™ 1]
Application
Recommended Usage
Immunoprecipitation
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Recommended applications are based on validated applications from the unconjugated base product MAB2857. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark. Do not freeze.
Background: Mast Cell Protease-11/Prss34
Alternate Names
Mast Cell Protease11, MCP-11, MCPT11, Prss34
Gene Symbol
PRSS33
Additional Mast Cell Protease-11/Prss34 Products
Product Documents for Mast Cell Protease-11/Prss34 Antibody (451421) [CoraFluor™ 1]
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Mast Cell Protease-11/Prss34 Antibody (451421) [CoraFluor™ 1]
Sold for research purposes only under agreement from Massachusetts General Hospital, US Patent No. 12,134,721. A license is required for development of new commercial assay platforms (e.g., microarrays, microfluidics), diagnostic use or other unlicensed uses. Please contact Technical Support for further details: techsupport@bio-techne.com.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- Immunoprecipitation Protocol
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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