Key Product Details

Species Reactivity

Mouse

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG1 Clone # 451421
View all Mast Cell Protease-11/Prss34 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Mast Cell Protease‑11/Prss34
Met20-Ser318
Accession # Q80UR4

Specificity

Detects mouse Mast Cell Protease‑11/Prss34 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse (rm) MCP-1, rmMCP-6, or rmMCP-7 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Applications for Mouse Mast Cell Protease‑11/Prss34 Antibody

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Mouse Mast Cell Protease‑11/Prss34 (Catalog # 2857-SE), see our available Western blot detection antibodies

Western Blot

1 µg/mL
Sample: Recombinant Mouse Mast Cell Protease‑11/Prss34 (Catalog # 2857-SE)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Mast Cell Protease-11/Prss34

Mast Cell Protease-11 (MCP-11) is encoded by Prss34, one of 13 genes on mouse chromosome 17A3.3 that correspond to functional trypsin-like serine proteases (1). The deduced amino acid sequence of mouse MCP-11 consists of 318 residues with a signal peptide (residues 1 to 19), a pro region (residue 20 to 34), and a catalytic domain (35 to 318). The mRNA is preferentially expressed in spleen and bone marrow. The amino acid sequence of mouse MCP-11 shares 84% and 60% identity with that of rat and dog, respectively. The human gene corresponding to Prss34 contains a mutation that leads to a premature translation termination codon, thus encoding a protein that is unlikely to be enzymatically active.

References

  1. Wong, G.W. et al. (2004) J. Biol. Chem. 279:2438.

Alternate Names

Mast Cell Protease11, MCP-11, MCPT11, Prss34

Entrez Gene IDs

328780 (Mouse)

Gene Symbol

PRSS34

UniProt

Q80UR4

Additional Mast Cell Protease-11/Prss34 Products

Product Documents for Mouse Mast Cell Protease‑11/Prss34 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse Mast Cell Protease‑11/Prss34 Antibody

For research use only

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