Max Antibody (4E10-1A9) - Azide and BSA Free

Novus Biologicals | Catalog # H00004149-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Proximity Ligation Assay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 4E10-1A9

Format

Azide and BSA Free
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Product Specifications

Immunogen

MAX (AAH03525, 1 a.a. ~ 151 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSDNDDIEVESDADKRAHHNALERKRRDHIKDSFHSLRDSVPSLQGEKASRAQILDKATEYIQYMRRKNHTHQQDIDDLKRQNALLEQQVRALEKARSSAQLQTNYPSSDNSLYTNAKGSTISAFDGGSDSSSESEPEEPQSRKKLRMEAS

Localization

Nuclear

Specificity

MAX - MYC associated factor X

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Max Antibody (4E10-1A9) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Max Antibody (4E10-1A9) [H00004149-M01]

Immunocytochemistry/ Immunofluorescence: Max Antibody (4E10-1A9) [H00004149-M01]

Immunocytochemistry/Immunofluorescence: Max Antibody (4E10-1A9) [H00004149-M01] - Analysis of monoclonal antibody to MAX on HeLa cell. Antibody concentration 10 ug/ml.
Proximity Ligation Assay: Max Antibody (4E10-1A9) [H00004149-M01]

Proximity Ligation Assay: Max Antibody (4E10-1A9) [H00004149-M01]

Proximity Ligation Assay: Max Antibody (4E10-1A9) [H00004149-M01] - Analysis of protein-protein interactions between MAPK14 and MAX. HeLa cells were stained with anti-MAPK14 rabbit purified polyclonal 1:1200 and anti-MAX mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
ELISA: Max Antibody (4E10-1A9) [H00004149-M01]

ELISA: Max Antibody (4E10-1A9) [H00004149-M01]

ELISA: Max Antibody (4E10-1A9) [H00004149-M01] - Max Antibody (4E10-1A9) [H00004149-M01] - Detection limit for recombinant GST tagged MAX is approximately 0.1ng/ml as a capture antibody.

Applications for Max Antibody (4E10-1A9) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Reviewed Applications

Read 1 review rated 5 using H00004149-M01 in the following applications:

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Max

The protein encoded by this gene is a member of the basic helix-loop-helix leucine zipper (bHLHZ) family of transcription factors. It is able to form homodimers and heterodimers with other family members, which include Mad, Mxi1 and Myc. Myc is an oncoprotein implicated in cell proliferation, differentiation and apoptosis. The homodimers and heterodimers compete for a common DNA target site (the E box) and rearrangement among these dimer forms provides a complex system of transcriptional regulation. Multiple alternatively spliced transcript variants have been described for this gene but the full-length nature for some of them is unknown.

Long Name

Myc associated factor X

Alternate Names

BHLHD4, bHLHd4MGC11225, bHLHd5, bHLHd6, bHLHd7, bHLHd8, Class D basic helix-loop-helix protein 4, helix-loop-helix zipper protein, MAX protein, MGC10775, MGC18164, MGC34679, MGC36767, MYC associated factor X, Myc-associated factor X, orf1, protein max

Entrez Gene IDs

4149 (Human)

Gene Symbol

MAX

OMIM

154950 (Human)

UniProt

AAH03525 (Human)

Additional Max Products

Product Documents for Max Antibody (4E10-1A9) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Max Antibody (4E10-1A9) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Max Antibody (4E10-1A9) - Azide and BSA Free (1)

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  • Max Antibody (4E1.0-1A9)
    Name: Julien COUTIER
    Application: Western Blot
    Sample Tested: Human keratinocytes
    Species: Human
    Verified Customer | Posted 08/30/2018
    Total protein extract, 50 ug per lane, membrane blocked with PBS-BSA 3%, antibody diluted at 1/1000 in PBS overnight at 4°C.
    Total protein extract, 50 ug per lane, membrane blocked with PBS-BSA 3%, antibody diluted at 1/1000 in PBS overnight at 4°C.
    Max Antibody (4E10-1A9) - Azide and BSA Free H00004149-M01

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Protocols

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FAQs

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